HIPPOCAMPAL DAMAGE AND KAINIC ACID INJECTION INDUCE A RAPID INCREASE IN MESSENGER-RNA FOR BDNF AND NGF IN THE RAT-BRAIN
HIPPOCAMPAL DAMAGE AND KAINIC ACID INJECTION INDUCE A RAPID INCREASE IN MESSENGER-RNA FOR BDNF AND NGF IN THE RAT-BRAIN
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DOI：
10.1016/0014-4886(91)90082-n
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发表时间：
1991-10-01
影响因子：
5.3
通讯作者：
PERSSON, H
中科院分区：
文献类型：
--
作者：
BALLARIN, M;ERNFORS, P;PERSSON, H
原位杂交和 Northern 印迹用于研究兴奋性刺激后大鼠大脑中神经生长因子家族成员的 mRNA 表达。单侧进针或向背侧海马注射生理盐水一小时后，注射同侧齿状回颗粒神经元和梨状皮层中脑源性神经营养因子 (BDNF) mRNA 的水平显着增加。相同的治疗还增加了同侧齿状回颗粒神经元中 NGF mRNA 的水平。插入针头或注射生理盐水后，BDNF 和 NGF mRNA 的快速增加是短暂的，随后同一脑区的 c-fos mRNA 增加。与针插入本身或注射盐水相比，单侧将红藻氨酸注射到背侧海马后7小时，同侧海马以及同侧额顶叶、梨状和周围皮质、杏仁复合体、屏状核和下丘脑腹内侧部的BDNF mRNA水平显着增加。对侧的这些大脑区域也出现了不太明显的增加。 Northern 印迹显示，与假手术对照动物相比，对侧海马和同侧海马中 BDNF mRNA 的水平分别增加了 5 倍和 40 倍。与 BDNF 和 NGF 相比，海马源性神经营养因子/神经营养因子-3 (HDNF/NT-3) mRNA 的水平不会因针插入或注射盐水或红藻氨酸而改变。这些结果表明海马损伤差异性地诱导大脑中神经营养因子 NGF 家族的表达，并进一步表明大脑中 BDNF mRNA 的表达因突触活动的增加而显着增强。
In situhybridization and Northern blots were used to study expression of mRNAs for members of the nerve growth factor family in the rat brain following an excitatory stimulus. One hour after a unilateral needle insertion or saline injection into the dorsal hippocampus, the level of brain-derived neurotrophic factor (BDNF) mRNA increased markedly in granular neurons of the dentate gyrus and in the piriform cortex ipsilateral to the injection. The same treatment also increased the level of NGF mRNA in granular neurons of the ipsilateral dentate gyrus. The rapid increase in BDNF and NGF mRNA after a needle insertion or injection of saline was transient and preceded by an increase in c-fos mRNA in the same brain regions. In contrast to a needle insertion per se or a saline injection, 7 h after a unilateral injection of kainic acid into the dorsal hippocampus, the level of BDNF mRNA was dramatically increased in the ipsilateral hippocampus, as well as in the ipsilateral frontoparietal, piriform and perihinal cortex, the amygdaloid complex, claustrum, and ventromedial hypothalamus. A less pronounced increase was also seen in these brain areas on the contralateral side. Northern blots revealed that the level of BDNF mRNA increased 5- and 40-fold in the contra- and ipsilateral hippocampus, respectively, compared to sham-operated control animals. In contrast to BDNF and NGF, the level of hippocampus-derived neurotrophic factor/neurotrohin-3 (HDNF/NT-3) mRNA was not altered by either needle insertion or injection of saline or kainic acid. These results indicate that hippocampal damage differentially induces expression of the NGF family of neurotrophic factors in the brain and suggest further that BDNF mRNA expression in the brain is markedly enhanced by an increased synaptic activity.