Synthetic approaches to protein phosphorylation.
Synthetic approaches to protein phosphorylation.
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DOI：
10.1016/j.cbpa.2015.07.001
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发表时间：
2015-10
影响因子：
7.8
通讯作者：
Cole PA
中科院分区：
文献类型：
--
作者：
Chen Z;Cole PA
可逆的蛋白质磷酸化在生物学和医学中至关重要。已经发现了数十万个蛋白质磷酸化位点，但我们对这些翻译后修饰的绝大多数功能缺乏了解。本文介绍了几种化学和生物化学方法，正在开发和目前使用的安装磷酸氨基酸和它们的模拟物位点特异性地进入蛋白质。全化学合成，半合成和无义抑制策略研究蛋白质磷酸化的相对优点进行了讨论，在技术简单性，范围和通用性。
Reversible protein phosphorylation is critically important in biology and medicine. Hundreds of thousands of sites of protein phosphorylation have been discovered but our understanding of the functions of the vast majority of these post-translational modifications is lacking. This review describes several chemical and biochemical methods that are under development and in current use to install phospho-amino acids and their mimics site-specifically into proteins. The relative merits of total chemical synthesis, semisynthesis, and nonsense suppression strategies for studying protein phosphorylation are discussed in terms of technical simplicity, scope, and versatility.