Studies of metaphase and interphase chromosomes using fluorescence in situ hybridization.
Studies of metaphase and interphase chromosomes using fluorescence in situ hybridization.
复制标题
使用荧光原位杂交研究中期和间期染色体。
DOI：
10.1101/sqb.1993.058.01.084
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Wu,M
中科院分区：
文献类型：
--
作者：
Trask,BJ;Allen,S;Massa,H;Fertitta,A;Sachs,R;vandenEngh,G;Wu,M
荧光原位杂交技术（FISH）可以直接研究中期和间期染色体的包装。使用这种方法，荧光信号在特定DNA序列（探针）的位置产生。利用不同的探针修饰策略，不同的DNA序列可以在染色体或细胞核中相互定位。在本文中，我们简要地回顾了这项技术，因为它对从原位结果推断天然的、未经处理的染色质的组织有潜在的影响。我们描述了间期和中期染色体序列的接近度如何与基因组距离（沿线性DNA分子以碱基对定义的距离）相关。我们比较了中期和间期染色体的分辨率。最后，我们讨论了这些发现对结合使用原位杂交和特定DNA序列检测染色体异常的影响。
The packaging of chromosomes in metaphase and interphase chromosomes can be directly studied by means of fluorescence in situ hybridization (FISH). With this method, a fluorescent signal is produced at the site of specific DNA sequences (probes). With different probe modification strategies, different DNA sequences can be localized with respect to one another in chromosomes or nuclei. In this paper, we briefly review the technique, because of its potential impact on the inference of the organization of native, untreated chromatin from in situ results. We describe how the proximity of sequences in interphase and metaphase chromosomes correlates with genomic distance (the distance defined in base pairs along the linear DNA molecule). We contrast the resolution that can be obtained in metaphase and interphase chromosomes. Finally, we discuss the impact of these findings on the combined use of in situ hybridization and specific DNA sequences to detect chromosome abnormalities.