TGF-β1 induced fascinl expression facilitates the migration and invasion of kidney carcinoma cells through ERK and JNK signaling pathways
TGF-β1 induced fascinl expression facilitates the migration and invasion of kidney carcinoma cells through ERK and JNK signaling pathways
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DOI：
10.1016/j.bbrc.2018.05.081
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发表时间：
2018-07-02
影响因子：
3.1
通讯作者：
Kong, Chuize
中科院分区：
文献类型：
--
作者：
Yang, Jianyu;Zhang, Naiwen;Kong, Chuize
转化生长因子-β1在肿瘤发展过程中控制细胞侵袭和运动能力的信号网络中起着至关重要的作用。采用实时荧光定量聚合酶链式反应和免疫印迹法检测转化生长因子-β1在769-P和OSRC细胞中的表达，以探讨其在转化生长因子-β1促进的肾癌细胞侵袭和迁移中的作用。利用小干扰RNA(SiRNA)技术沉默Fascin1。细胞骨架染色检测细胞骨架的变化。通过创面愈合和Transwell实验检测细胞迁移和侵袭的变化。结果表明，经10 ng/mlTGF-β1处理后，769-P细胞和OSRC细胞中ZONIL的mRNA和蛋白水平均显著升高。经10 ng/mlTGF-β1处理后，两种细胞均可发生上皮-间充质转化(EMT)，促进细胞的侵袭和迁移能力。此外，SINGIL siRNA显著减弱了由TGF-β1诱导的侵袭和迁移。此外，我们还发现ERK和INK信号通路的特异性抑制剂FR180204和SP600125可以抑制由TGF-β1诱导的SENIL的表达。综上所述，这些结果表明SENCEL是转化生长因子-β1通过ERK和JNK信号通路诱导KCC侵袭和迁移的重要介导物。(C)2018 Elsevier Inc.保留所有权利。
Transforming growth factor-beta 1 (TGF-beta 1) plays a crucial role in the signaling network that controls cellular invasion and motility capability during tumor development. To investigate whether fascin1 plays a crucial role in TGF-beta 1-facilitated invasion and migration of kidney cancer cells (KCC), real-time PCR and western blotting were used to test the fascin1 expression after TGF-beta 1 treatment (10 ng/ml) in 769-P and OSRC cells. Fascin1 was silenced using the small interfering RNA (siRNA) technique. Cytoskeleton staining was used to test the change of Cytoskeleton. Cell migration and invasion changes were measured by wound-healing and Transwell assay. The results indicate that mRNA and protein levels of fascinl were dramatically increased after treatment with 10 ng/ml TGF-beta 1 in 769-P and OSRC cells. TGF-beta 1 promoted the occurrence of EMT (Epithelial-Mesenchymal Transition) and the invasive and migratory capabilities of the two cell lines after treatment with 10 ng/ml TGF-beta 1. In addition, fascinl siRNA dramatically attenuated the invasiveness and migration induced by TGF-beta 1. Furthermore, we identified that specific inhibitors of ERK and INK signaling pathways, FR180204 and SP600125, can suppress TGF-beta 1-induced fascinl expression. In conclusion, these results reveal that fascinl is an important mediator of TGF-beta 1-induced invasion and migration of KCC through ERK and JNK signal pathways. (C) 2018 Elsevier Inc. All rights reserved.