The Efficacy of Shikonin on Cartilage Protection in a Mouse Model of Rheumatoid Arthritis
The Efficacy of Shikonin on Cartilage Protection in a Mouse Model of Rheumatoid Arthritis
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DOI：
10.4196/kjpp.2010.14.4.199
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发表时间：
2010-08-01
影响因子：
2
通讯作者：
Yim, Sung-Vin
中科院分区：
文献类型：
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作者：
Kim, Young Ock;Hong, Seung Jae;Yim, Sung-Vin
探讨紫草素对类风湿性关节炎（RA）的治疗作用。作为RA动物模型，DBA/1 J小鼠用II型胶原免疫两次。第二次胶原免疫后，小鼠口服紫草素（2 mg/kg），每日1次，连续35 d，观察其发病率、临床评分、骨密度（BMD）、骨矿含量（BMC）及关节组织病理学变化。与对照组相比，紫草素治疗组胫骨近端区域的BMD大幅增加。我们还研究了紫草素对炎性细胞因子和软骨保护的影响。紫草素治疗显着降低胶原诱导性关节炎（CIA）的发病率和严重程度，显着消除关节肿胀和软骨破坏。紫草素还能显著抑制CIA小鼠基质金属蛋白酶（MMP）-1的产生，上调基质金属蛋白酶组织抑制因子（TIMP）-1。总之，紫草素通过调节MMP/TIMP发挥治疗作用，这些结果表明，紫草素是一个优秀的候选人作为软骨保护药物的RA。
The potential therapeutic action of shikonin in an experimental model of rheumatoid arthritis (RA) was investigated. As a RA animal model, DBA/1J mice were immunized two times with type II collagen. After the second collagen immunization, mice were orally administered shikonin (2 mg/kg) once a day for 35 days, and the incidence, clinical score, bone mineral density (BMD), bone mineral content (BMC) and joint histopathology were evaluated. BMD in the proximal regions of the tibia largely increased in the shikonin treatment group compared with the control group. We also examined the effect of shikonin on inflammatory cytokines and cartilage protection. Shikonin treatment significantly reduced the incidence and severity of collagen-induced arthritis (CIA), markedly abrogating joint swelling and cartilage destruction. Shikonin also significantly inhibited the production of matrix metalloproteinase (MMP)-1 and up-regulated tissue inhibitors of metalloproteinase (TIMP)-1 in mice with CIA. In conclusion, shikonin exerted therapeutic effects through regulation of MMP/TIMP; these results suggest that shikonin is an outstanding candidate as a cartilage protective medicine for RA.