Deep Sequencing Identifies Viral and Wasp Genes with Potential Roles in Replication of Microplitis demolitor Bracovirus
Deep Sequencing Identifies Viral and Wasp Genes with Potential Roles in Replication of Microplitis demolitor Bracovirus
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DOI：
10.1128/jvi.06434-11
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发表时间：
2012-03-01
影响因子：
5.4
通讯作者：
Strand, Michael R.
中科院分区：
文献类型：
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作者：
Burke, Gaelen R.;Strand, Michael R.
Bracovirus（BV）（Polydnaviridae）属中的病毒是寄生蜂的共生体，其特异性地在雌性的卵巢中复制。最近的分析表达的序列标签从两个黄蜂物种，Cotesia congregata和Chelonus inanitus，确定了24个不同的nudivirus基因相关的成绩单。这些结果与其他数据一起强烈地表明，BV从裸尾鱼的祖先进化而来。然而，目前尚不清楚携带BV的黄蜂是否含有其他nudivirus样基因，以及BV复制可能还需要哪些类型的黄蜂基因。红侧沟茧蜂携带红侧沟茧蜂杆状病毒（MdBV）。在这里，我们表征了MdBV复制并进行了M.拆除卵巢转录本。我们的研究结果表明，MdBV复制开始于第2阶段的脓疱，并继续在成人。分析复制前和活跃复制阶段的卵巢RNA产生了22 Gb的序列，组装成66，425个转录本。如此广泛的取样表明，M.拆除者基因组测序。共鉴定出41个裸病毒样转录物，其中大多数在MdBV复制期间高度表达。我们的研究结果还确定了一套黄蜂基因，在MdBV复制过程中高度表达。在这些产物中，有几种转录本在真核生物调节基因座特异性DNA扩增中具有保守作用。总的来说，我们的数据集与先前的结果可能确定大多数nudivirus相关的基因，在BV复制过程中的转录功能。我们的研究结果还表明，前病毒DNA的扩增包装成BV病毒粒子可能取决于黄蜂的复制机制。
Viruses in the genus Bracovirus (BV) (Polydnaviridae) are symbionts of parasitoid wasps that specifically replicate in the ovaries of females. Recent analysis of expressed sequence tags from two wasp species, Cotesia congregata and Chelonus inanitus, identified transcripts related to 24 different nudivirus genes. These results together with other data strongly indicate that BVs evolved from a nudivirus ancestor. However, it remains unclear whether BV-carrying wasps contain other nudivirus-like genes and what types of wasp genes may also be required for BV replication. Microplitis demolitor carries Microplitis demolitor bracovirus (MdBV). Here we characterized MdBV replication and performed massively parallel sequencing of M. demolitor ovary transcripts. Our results indicated that MdBV replication begins in stage 2 pupae and continues in adults. Analysis of prereplication- and active-replication-stage ovary RNAs yielded 22 Gb of sequence that assembled into 66,425 transcripts. This breadth of sampling indicated that a large percentage of genes in the M. demolitor genome were sequenced. A total of 41 nudivirus-like transcripts were identified, of which a majority were highly expressed during MdBV replication. Our results also identified a suite of wasp genes that were highly expressed during MdBV replication. Among these products were several transcripts with conserved roles in regulating locus-specific DNA amplification by eukaryotes. Overall, our data set together with prior results likely identify the majority of nudivirus-related genes that are transcriptionally functional during BV replication. Our results also suggest that amplification of proviral DNAs for packaging into BV virions may depend upon the replication machinery of wasps.