Microfluidics-Produced Collagen Fibers Show Extraordinary Mechanical Properties
Microfluidics-Produced Collagen Fibers Show Extraordinary Mechanical Properties
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DOI：
10.1021/acs.nanolett.6b02828
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发表时间：
2016-09-01
期刊：
影响因子：
10.8
通讯作者：
Scheibel, Thomas
中科院分区：
文献类型：
--
作者：
Haynl, Christian;Hofmann, Eddie;Scheibel, Thomas
胶原蛋白因其生物可降解性、生物相容性和低致敏性而被广泛应用于药物传递和组织工程领域。除明胶基材料外，胶原蛋白微纤维是生物医学研究的热点。一般;通过湿纺丝生产出直径大于8 μ m的人造纤维。本研究利用微流控芯片建立了组装和连续生产单一胶原I型微纤维的方法。微流体制造的微纤维表现出：抗拉强度和杨氏模量——超过了经典湿纺丝装置生产的纤维，甚至超过了天然肌腱，而且它们的直径更小。通过偏振傅里叶变换红外光谱（FTIR）检测了它们的结构取向，显示了纤维在微纤维内的取向。使用神经细胞系NG108-15进行的细胞培养测试显示，细胞沿微纤维轴排列和轴突生长，开辟了在周围神经修复等方面的潜在应用。
Collagens arc widely used as biomaterials in drug-delivery and tissue engineering applications due to their biodegradability, biocompatibility and hypoallergenicity. sides gelatin-based materials, collagen microfibers are in the focus of biomedical research. Commonly; man-made fibers are produced by wet-spinning yielding fiber diameters higher than 8 mu m. Here, assembly and continuous,production of single collagen type I microfibers were established using a microfluidic chip. Microfluidics-produced microfibers exhibited :tensile strength and Young's modulus-exceeding that of fibers produced in classical wet-spinning devices and even that of natural tendon and they showed lower diameters. Their structural orientation was examined by polarized Fourier transform infrared spectroscopy (FTIR) showing fibril alignment within the, microfiber. Cell culture tests using the neuronal-cell line NG108-15 showed cell alignment and axon growth along the microfiber axes inaugurating potential applications in, for example, peripheral nerve repair.