Detection of immune complexes by a new assay, the polyethylene glycol precipitation-complement consumption test (PEG-CC).
Detection of immune complexes by a new assay, the polyethylene glycol precipitation-complement consumption test (PEG-CC).
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通过新的检测方法——聚乙二醇沉淀-补体消耗试验（PEG-CC）来检测免疫复合物。
DOI：
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发表时间：
1979
影响因子：
4.6
通讯作者：
D. Brown
中科院分区：
文献类型：
--
作者：
G. Harkiss;D. Brown
一种新的检测循环免疫复合物的方法，聚乙二醇沉淀-补体消耗法（PEG-CC）。该方法简便、灵敏，具有高度的特异性。免疫复合物首先通过2.5%聚乙二醇（PEG）沉淀从血清中分离出来并浓缩。然后通过使用敏感的溶血补体动力学测定法测量它们固定补体的能力来测定它们的功能。该试验可检测血清中聚集的IgG，浓度约为6.0微克/毫升（绝对浓度约为2.0微克）。采用体外制备的dna -抗dna和卵清蛋白-抗卵清蛋白免疫复合物，抗原检测浓度小于0.5微克/毫升。内源性补体、多阴离子和其他因素在测试血清中的干扰实际上已经被检测设计消除了。PEG-CC检测免疫复合物特异性的提高，将有助于免疫复合物介导疾病的诊断和监测。
A new assay for the detection of circulating immune complexes, the polyethylene glycol precipitation-complement consumption assay (PEG-CC), is described. The test is both simple and sensitive, and exhibits a high degree of specificity. Immune complexes are first isolated from serum by precipitation in 2.5% polyethylene glycol (PEG) and concentrated. They are then assayed functionally by measuring their ability to fix complement using a sensitive kinetic assay for total haemolytic complement. The test can detect aggregated IgG in serum at concentrations around 6.0 micrograms/ml (about 2.0 micrograms absolute. Using DNA-anti-DNA and ovalbumin-anti-ovalbumin immune complexes prepared in vitro, antigen concentrations less than 0.5 micrograms/ml can be detected. Interference by endogenous complement, polyanions and other factors in test sera has been virtually eliminted by the design of the assay. The increased specificity of the PEG-CC test for immune complexes, should prove useful in the diagnosis and monitoring of immune complex-mediated diseases.