Optogenetically controlled protein kinases for regulation of cellular signaling.
Optogenetically controlled protein kinases for regulation of cellular signaling.
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DOI：
10.1039/c7cs00404d
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发表时间：
2018-04-03
影响因子：
46.2
通讯作者：
Verkhusha VV
中科院分区：
文献类型：
--
作者：
Leopold AV ;Chernov KG ;Verkhusha VV
蛋白激酶参与许多细胞过程的调节，包括细胞分化、存活、迁移、轴突导向和神经元可塑性。越来越多的光遗传学工具，称为光激酶，允许用光激活和抑制不同的蛋白激酶。光遗传学调控使得能够快速、可逆和非侵入性地操纵蛋白激酶活性，补充传统方法，例如用生长因子、蛋白激酶抑制剂或化学二聚化剂治疗。本文综述了目前控制酪氨酸激酶和丝氨酸-苏氨酸激酶的光遗传学工具的特点。我们讨论了如何光激酶可以应用于蛋白激酶信号在细胞和生物体的空间和时间方面的研究。我们比较了化学和光遗传学调节蛋白激酶活性的方法，并提出了选择光激酶和用光控制它们的设备的指导方针。我们还描述了各种光感受器的基础上，工程师新的光激酶的策略。
Protein kinases are involved in the regulation of many cellular processes including cell differentiation, survival, migration, axon guidance and neuronal plasticity. A growing set of optogenetic tools, termed opto-kinases, allows activation and inhibition of different protein kinases with light. The optogenetic regulation enables fast, reversible and non-invasive manipulation of protein kinase activities, complementing traditional methods, such as treatment with growth factors, protein kinase inhibitors or chemical dimerizers. In this review, we summarize properties of the existing optogenetic tools for controlling of tyrosine kinases and serine-threonine kinases. We discuss how the opto-kinases can be applied for studies of spatial and temporal aspects of protein kinase signaling in cells and organisms. We compare approaches for chemical and optogenetic regulation of protein kinase activity and present guidelines for selection of opto-kinases and equipment to control them with light. We also describe strategies to engineer novel opto-kinases on the basis of various photoreceptors.
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影响因子：
--
作者：
Christian, Jan L.
通讯作者：
Christian, Jan L.
DOI：
10.1126/science.aah3404
发表时间：
2016-12-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dagliyan O;Tarnawski M;Chu PH;Shirvanyants D;Schlichting I;Dokholyan NV;Hahn KM
通讯作者：
Hahn KM
影响因子：
16.6
作者：
Bugaj LJ;Spelke DP;Mesuda CK;Varedi M;Kane RS;Schaffer DV
通讯作者：
Schaffer DV
DOI：
10.1073/pnas.1218319110
发表时间：
2013-04-23
影响因子：
11.1
作者：
Dagliyan, Onur;Shirvanyants, David;Dokholyan, Nikolay V.
通讯作者：
Dokholyan, Nikolay V.
影响因子：
4.6
作者：
Chatelle CV;Hövermann D;Müller A;Wagner HJ;Weber W;Radziwill G
通讯作者：
Radziwill G