Short-term effects of salt stress on antioxidant systems and leaf water relations of pea leaves
Short-term effects of salt stress on antioxidant systems and leaf water relations of pea leaves
复制标题
DOI：
10.1034/j.1399-3054.2002.1150211.x
复制
发表时间：
2002-06-01
影响因子：
6.4
通讯作者：
Almansa, MS
中科院分区：
文献类型：
--
作者：
Hernández, JA;Almansa, MS
豌豆（Pisum sativum L.）研究了短期盐胁迫和恢复对植物生长、水分关系和抗氧化酶活性的影响。叶生长被盐中断。然而，在复苏期间，增长得到恢复，尽管恢复到控制水平有所延迟。盐胁迫导致渗透势和气孔导度下降，但在胁迫后48 h和24 h，这两个参数分别恢复到对照值。在豌豆叶中，在盐处理的植物中观察到Na+浓度的线性增加。在恢复的植物中，观察到Na+浓度略有降低，这可能是由于稀释效应，因为植物生长恢复，并且胁迫期后叶片中的总Na+含量保持不变。SOD活性在胁迫48 h后和恢复期8 h后显著增加（分别增加53%和42%），并在胁迫后24 h达到对照值。APX活性在胁迫期间没有变化，仅在胁迫后8 h，就比对照叶片增加了48%。GR在盐胁迫24 h后显示出71%的增加，并且在恢复的植物中也观察到显著增加。在胁迫8 h后观察到TBARS的强烈增加（增加180%），但随后在胁迫期间观察到迅速下降。令人惊讶的是，TBARS在胁迫后8小时再次增加（增加78%），表明植物可以在恢复的第一个小时期间将NaCl从水培培养物中的消除视为另一种胁迫。这些结果表明，短期NaCl胁迫对生长，叶片水分关系和SOD和APX活性产生可逆的影响。这项工作还表明，在施加压力的最初几个小时和恢复的最初几个小时内，都产生了氧化应激。
In pea (Pisum sativum L.) plants the effect of short-term salt stress and recovery on growth, water relations and the activity of some antioxidant enzymes was studied. Leaf growth was interrupted by salt addition. However, during recovery, growth was restored, although there was a delay in returning to control levels. Salt stress brought about a decrease in osmotic potential and in stomatal conductance, but at 48 h and 24 h post-stress, respectively, both parameters recovered control values. In pea leaves, a linear increase in the Na+ concentration was observed in salt treated plants. In the recovered plants, a slight reduction in the Na+ concentration was observed, probably due to a dilution effect since the plant growth was restored and the total Na+ content was maintined in leaves after the stress period. A significant increase of SOD activity occurred after 48 h of stress and after 8 h of the recovery period (53% and 42%, respectively), and it reached control values at 24 h post-stress. APX activity did not change during the stress period, and after only 8 h post-stress it was increased by 48% with respect to control leaves. GR showed a 71% increase after 24 h of salt stress and also a significant increase was observed in the recovered plants. A strong increase of TBARS was observed after 8 h of stress (180% increase), but then a rapid decrease was observed daring the stress period. Surprisingly, TBARS again increased at 8 h post-stress (78% increase), suggesting that plants could perceive the elimination of NaCl from the hydroponic cultures as another stress during the first hours of recovery. These results suggest that short-term NaCl stress produces reversible effects on growth, leaf water relations and on SOD and APX activities. This work also suggests that both during the first hours of imposition of stress and during the first hours of recovery an oxidative stress was produced.