Novel in vivo techniques to visualize kidney anatomy and function.
Novel in vivo techniques to visualize kidney anatomy and function.
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DOI：
10.1038/ki.2015.65
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发表时间：
2015-07
影响因子：
19.6
通讯作者：
中科院分区：
文献类型：
--
作者：
过去几年，使用多光子显微镜（MPM）进行活体成像已成为肾脏研究中越来越流行和广泛使用的实验技术。 MPM 能够以亚微米分辨率对完整的活体肾组织进行深度光学切片，这在活体成像方法中是无与伦比的。 MPM 解决了肾脏研究中长期存在的关键技术障碍，以在其天然环境中研究几种复杂且难以接近的细胞类型和体内解剖结构。全面、定量的肾脏结构和功能 MPM 研究有助于我们更好地了解健康和患病肾脏的细胞和分子机制。本综述总结了最近的体内 MPM 研究，重点关注肾小球和滤过屏障，尽管也提到了与肾小球相关的肾血管和肾小管功能。讨论了在肾小球疾病进展过程中连续几天在完整肾脏中对同一肾小球进行连续 MPM 的最新应用。这种视觉方法与细胞谱系的基因编码荧光标记相结合，有助于追踪肾小球病理发展过程中单个足细胞的命运和功能（例如细胞钙变化），并为肾小球环境的高度动态而非静态性质提供视觉证据。未来的活体成像应用有望进一步突破光学显微镜的极限，并增进我们对肾损伤机制的理解。此外，MPM 将有助于研究组织修复和再生的新机制，这是肾脏研究的前沿领域。
Intravital imaging using multiphoton microscopy (MPM) has become an increasingly popular and widely used experimental technique in kidney research over the past few years. MPM allows deep optical sectioning of the intact, living kidney tissue with submicron resolution which is unparalleled among intravital imaging approaches. MPM has solved a long-standing critical technical barrier in renal research to study several complex and inaccessible cell types and anatomical structures in vivo in their native environment. Comprehensive and quantitative kidney structure and function MPM studies helped our better understanding of the cellular and molecular mechanisms of the healthy and diseased kidney. This review summarizes recent in vivo MPM studies with a focus on the glomerulus and the filtration barrier, although select, glomerulus-related renal vascular and tubular functions are also mentioned. The latest applications of serial MPM of the same glomerulus in vivo, in the intact kidney over several days, during the progression of glomerular disease are discussed. This visual approach, in combination with genetically encoded fluorescent markers of cell lineage, has helped to track the fate and function (e.g. cell calcium changes) of single podocytes during the development of glomerular pathologies, and provided visual proof for the highly dynamic rather than static nature of the glomerular environment. Future intravital imaging applications have the promise to further push the limits of optical microscopy, and to advance our understanding of the mechanisms of kidney injury. Also, MPM will help to study new mechanisms of tissue repair and regeneration, a cutting edge area of kidney research.
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影响因子：
3.7
作者：
Han Z;Jin L;Chen F;Loturco JJ;Cohen LB;Bondar A;Lazar J;Pieribone VA
通讯作者：
Pieribone VA
DOI：
10.1152/ajprenal.00521.2005
发表时间：
2006-08-01
影响因子：
4.2
作者：
Kang, Jung Julie;Toma, Ildiko;Peti-Peterdi, Janos
通讯作者：
Peti-Peterdi, Janos
DOI：
10.1159/000360673
发表时间：
2014
期刊：
Nephron. Experimental nephrology
影响因子：
--
作者：
Peti-Peterdi J;Burford JL;Hackl MJ
通讯作者：
Hackl MJ
影响因子：
19.6
作者：
通讯作者：
--
影响因子：
5.3
作者：
Arosio D;Ratto GM
通讯作者：
Ratto GM