Identification and characterization of novel recombinant vaccine antigens for immunization against genital Chlamydia trachomatis.
Identification and characterization of novel recombinant vaccine antigens for immunization against genital Chlamydia trachomatis.
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DOI：
10.1111/j.1574-695x.2008.00527.x
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发表时间：
2009-03
影响因子：
--
通讯作者：
Reed SG
中科院分区：
文献类型：
--
作者：
Coler RN;Bhatia A;Maisonneuve JF;Probst P;Barth B;Ovendale P;Fang H;Alderson M;Lobet Y;Cohen J;Mettens P;Reed SG
Chlamydia trachomatis infection is the most common sexually transmitted bacterial infection worldwide with over 91 million cases estimated annually. An effective subunit vaccine against Chlamydia may require a multivalent subunit cocktail of antigens in a single formulation for broad coverage of a heterogeneous MHC population. Herein we describe the identification by CD4+ and CD8+ T cell expression cloning, serological expression cloning, and an in silico analysis of the C. trachomatis genome, of novel C. trachomatis antigens. These antigens elicited human CD4+ T cell responses, and a subset proved to be immunogenic and protective when administered as immunoprophylactic vaccines against C. trachomatis challenge. Candidate vaccines consisting of the prioritized C. trachomatis antigens adjuvanted in GSK proprietary AS01B adjuvant were prioritized based on induction of solid protection against challenge in C57BL/6 and BALB/c mice with C. trachomatis. Some of the vaccines prevented bacterial shedding and colonization of the upper genital tract to varying degrees by mechanisms that may include CD4+ T cells.
