XA23 Is an Executor R Protein and Confers Broad-Spectrum Disease Resistance in Rice
XA23 Is an Executor R Protein and Confers Broad-Spectrum Disease Resistance in Rice
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XA23 是一种执行 R 蛋白，赋予水稻广谱抗病性
DOI：
10.1016/j.molp.2014.10.010
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发表时间：
2015-02-02
期刊：
影响因子：
27.5
通讯作者：
Zhao, Kaijun
中科院分区：
文献类型：
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作者：
Wang, Chunlian;Zhang, Xiaoping;Zhao, Kaijun
大多数植物抗病基因编码的蛋白质具有共同的结构特征。然而，转录激活因子样效应（TALE）相关的执行子型R基因与任何已知的R基因没有相当大的序列同源性。我们采用基于图谱的克隆方法和基于tale的技术，分离并鉴定了来自野生水稻（Oryza rufipogon）的一个新的执行R基因Xa23，该基因具有极广谱的抗黄单胞菌（Xanthomonas otyzae pv）白叶枯病的抗性。otyzae (Xoo语)。Xa23编码一个113个氨基酸的蛋白，与已知的执行子R蛋白XA10有50%的相似性。预测的XA23的跨膜螺旋也与XA10的重叠。然而，与Xa10不同的是，Xa23的转录被AvrXa23特异性激活，AvrXa23是所有检测的Xoo野株中存在的TALE。此外，易感等位基因xa23具有与xa23相同的开放阅读框，但由于缺乏AvrXa23的TALE结合元件（EBE），在启动子区域存在差异。XA23可以在水稻、烟草和番茄中引发强烈的过敏反应。我们的研究结果首次证明了植物基因组中存在一个执行R基因家族，该家族成员通过识别病原体中的同源tale来执行其功能和抗病谱。
The majority of plant disease resistance (R) genes encode proteins that share common structural features. However, the transcription activator-like effector (TALE)-associated executor type R genes show no considerable sequence homology to any known R genes. We adopted a map-based cloning approach and TALE-based technology to isolate and characterize Xa23, a new executor R gene derived from wild rice (Oryza rufipogon) that confers an extremely broad spectrum of resistance to bacterial blight caused by Xanthomonas otyzae pv. otyzae (Xoo). Xa23 encodes a 113 amino acid protein that shares 50% identity with the known executor R protein XA10. The predicted transmembrane helices in XA23 also overlap with those of XA10. Unlike Xa10, however, Xa23 transcription is specifically activated by AvrXa23, a TALE present in all examined Xoo field isolates. Moreover, the susceptible xa23 allele has an identical open reading frame of Xa23 but differs in promoter region by lacking the TALE binding element (EBE) for AvrXa23. XA23 can trigger a strong hypersensitive response in rice, tobacco, and tomato. Our results provide the first evidence that plant genomes have an executor R gene family of which members execute their function and spectrum of disease resistance by recognizing the cognate TALEs in the pathogen.