Time-resolved fluorescence spectroscopy for illuminating complex systems
Time-resolved fluorescence spectroscopy for illuminating complex systems
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DOI：
10.1016/s0003-2670(03)00723-2
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发表时间：
2003-12-19
影响因子：
6.2
通讯作者：
Munson, CA
中科院分区：
文献类型：
--
作者：
Bright, FV;Munson, CA
多年来，荧光团的发射特性（光谱，时间和偏振）已被广泛用于探测各种各样的系统。荧光寿命和旋转重定向时间测量，特别是，提供了一种手段来阐明复杂系统的关键细节。此外，因为荧光发生在纳秒（10（-9）s）时间尺度上，所以竞争或扰动动力学过程（如碰撞淬灭、溶剂弛豫、能量转移和旋转重取向）可以影响荧光，因此可以被量化。因此，一个精心选择和“放置”的荧光团可以作为一个报告员在广泛的纳秒或更快的事件。本文分为三节。理论部分讨论了时间分辨的各向异性和强度衰减动力学（时域和频域），泵浦探测光谱和上转换。第二部分介绍了时间相关单光子计数（TC-SPC）和多频相位调制荧光仪器。最后一节分为关于时间分辨荧光的使用的小节：（1）研究溶剂化动力学，生物化学系统，聚合物物理学和有组织的介质;（2）作为分离科学，显微镜和传感的工具;（3）与多光子激发策略相结合。(C)2003 Elsevier B.V保留所有权利。
Over the years, the emissive characteristics (spectral, temporal, and polarization) of fluorophores have been widely used to probe a wide variety of systems. Fluorescence lifetime and rotational reorientation time measurements, in particular, offer a means to elucidate key details about complex systems. Further, because fluorescence occurs on the nanosecond (10(-9) s) timescale, competing or perturbing kinetic processes like collisional quenching, solvent relaxation, energy transfer, and rotational reorientation can affect the fluorescence and hence be quantified. Thus, a carefully chosen and "placed" fluorophore can serve as an reporter on a wide range of nanosecond or faster events. This contribution is divided into three sections. The Theory section discusses time-resolved anisotropy and intensity decay kinetics (time and frequency domains), pump-probe spectroscopy, and up-conversion. The second section describes time-correlated single photon counting (TC-SPC) and multifrequency phase-modulation fluorescence instruments. The final section is divided into subsections on the use of time-resolved fluorescence: (1) to study solvation dynamics, biochemical systems, polymer photophysics, and organized media; (2) as a tool in the separation sciences, microscopy, and sensing; and (3) coupled with multiphoton excitation strategies. (C) 2003 Elsevier B.V All rights reserved.