PRODUCTION OF FETAL-LIKE ALKALINE PHOSPHATASE BY HELA CELLS
PRODUCTION OF FETAL-LIKE ALKALINE PHOSPHATASE BY HELA CELLS
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DOI：
10.1007/bf00485476
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发表时间：
1969-01-01
影响因子：
2.4
通讯作者：
COX, RP
中科院分区：
文献类型：
--
作者：
ELSON, NA;COX, RP
HeLa细胞产生的碱性磷酸酶在化学和物理性质上不同于成人器官和组织中的酶(Cox和Griffin，1967)。在目前的研究中，HeLa细胞碱性磷酸酶与人类胎盘中发现的一种胎儿形式的酶进行了比较。根据蔗糖密度梯度判断，这两种酶的相对分子质量大致相同，并且这些碱性磷酸酶的化学和物理性质相似。HeLa细胞酶的电泳谱与杂合子FS表型的胎盘碱性磷酸酶相似，只是运动较慢。使用抗HeLa细胞碱性磷酸酶抗体和胎盘和HeLa细胞酶作为抗原的双向免疫扩散显示这两种酶之间只有一条线的部分同一性，一个小的支点表明HeLa细胞酶上有额外的抗原位点。这些数据表明，HeLa培养的恶性细胞可能通过“基因组去抑制”产生一种类似胎儿的碱性磷酸酶。然而，这种酶的电泳学和免疫学特性发生了充分的变化，以至于它可以与正常生产的胎儿酶区分开来。
Alkaline phosphatase produced by HeLa cells differs in its chemical and physical properties from the enzyme found in adult organs and tissues (Cox and Griffin, 1967). In the present study HeLa cell alkaline phosphatase was compared to a fetal form of the enzyme found in human placenta. Both enzymes have approximately the same molecular weight as judged by sucrose density gradients, and the chemical and physical properties of these alkaline phosphatases are similar. The electrophoretic pattern of the HeLa cell enzyme resembles the placental alkaline phosphatase of the heterozygous FS phenotype except that it is slower moving. Double immunodiffusion using an antibody against HeLa cell alkaline phosphatase and placental and HeLa cell enzymes as antigens shows a single line of partial identity between the two enzymes, with a small spur suggesting additional antigenic sites on the HeLa cell enzyme. The data suggest that malignant cells in culture, HeLa, are producing a fetal-like alkaline phosphatase probably by “derepression of the genome.” However, the electrophoretic and immunological characteristics of the enzyme are altered sufficiently so that it can be distinguished from the normally produced fetal enzyme.