RhlR expression in Pseudomonas aeruginosa is modulated by the Pseudomonas quinolone signal via PhoB-dependent and -independent pathways
RhlR expression in Pseudomonas aeruginosa is modulated by the Pseudomonas quinolone signal via PhoB-dependent and -independent pathways
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DOI：
10.1128/jb.01378-06
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发表时间：
2006-12-01
影响因子：
3.2
通讯作者：
Haeussler, Susanne
中科院分区：
文献类型：
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作者：
Jensen, Vanessa;Loens, Dagmar;Haeussler, Susanne
铜绿假单胞菌的毒力决定因子的表达受到社会环境（通常称为群体感应）和环境信号的协调调节。在这项研究中，我们分析了花青素生产的各种独立调控水平，花青素生产受高丝氨酸内酯和喹诺酮假单胞菌信号（PQS）介导的群体感应系统以及铁和磷酸盐可用性的影响。我们证明，在磷酸盐限制下，磷酸盐调控子参与了rhlR的转录激活和PQS和pyocyanin生产的增加。然而，我们也观察到在低铁培养基条件下和添加独立于磷酸盐调节子的PQS后，rhlR转录增强。这些结果突出了铜绿假单胞菌通过环境线索和群体感应系统产生次生代谢物的复杂性。
The expression of virulence determinants in Pseudomonas aeruginosa is coordinately regulated in response to both the social environment-commonly referred to as quorum sensing-and to environmental cues. In this study we have dissected the various independent regulation levels for pyocyanin production, which is influenced by the homoserine lactone- and Pseudomonas quinolone signal (PQS)-mediated quorum-sensing systems as well as by iron and phosphate availability. We demonstrate that the phosphate regulon is involved in the transcriptional activation of rhlR and the augmentation of PQS and pyocyanin production under phosphate limitation. However, we also observed an enhancement of rhlR transcription under low-iron medium conditions and after the addition of PQS that was independent of the phosphate regulon. These results highlight the complexity of secondary metabolite production in P. aeruginosa via environmental cues and the quorum-sensing system.