Gene and Protein Expression of Cartilage Oligomeric Matrix Protein Associated with Oncogenesis in Canine Tumors
Gene and Protein Expression of Cartilage Oligomeric Matrix Protein Associated with Oncogenesis in Canine Tumors
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DOI：
10.1292/jvms.71.499
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发表时间：
2009-04-01
影响因子：
1.2
通讯作者：
Misumi, Kazuhiro
中科院分区：
文献类型：
--
作者：
Yamanokuchi, Kazuto;Yabuki, Akira;Misumi, Kazuhiro
为探讨软骨寡聚基质蛋白（COMP）的表达与肿瘤发生的关系，从25只患肿瘤的狗的血清和肿瘤组织中获得样品。肿瘤犬血清COMP水平显著高于正常犬，并与肿瘤组织提取物浓度相关。在肿瘤提取物的免疫印迹上检测到COMP抗体阳性手。免疫组化和原位杂交结果显示，COMP及其mRNA在乳腺肿瘤细胞胞浆中呈阳性信号，而在正常乳腺组织中未见阳性信号。在肥大细胞瘤和黑色素瘤细胞中也获得了COMP的阳性免疫组织化学结果。肿瘤发生可能增加犬血清COMP水平和COMP的产生。
To investigate the expression of cartilage oligomeric matrix protein (COMP) associated with oncogenesis, samples of serum and tumor tissue were obtained from 25 dogs with tumors. The serum levels of COMP in dogs with tumors were significantly higher than in 38 normal controls and correlated with the concentrations Of tumor tissue extracts. Positive hands to COMP antibody were detected on immunoblots of tumor extracts. Immunohistochemistry and in Situ hybridization demonstrated positive signals of COMP and its mRNA in the cytoplasm of tumor cells from mammary gland tumors, but not in the normal mammary gland. Positive immunohistochemistry results were also obtained for COMP in mast cell tumor and melanoma cells. Oncogenesis might augment production of COMP and the serum level of COMP in dogs.