Identifying macrophage enrichment in atherosclerotic plaques by targeting dual-modal US imaging/MRI based on biodegradable Fe-doped hollow silica nanospheres conjugated with anti-CD68 antibody
Identifying macrophage enrichment in atherosclerotic plaques by targeting dual-modal US imaging/MRI based on biodegradable Fe-doped hollow silica nanospheres conjugated with anti-CD68 antibody
复制标题
基于与抗 CD68 抗体偶联的可生物降解的掺铁空心二氧化硅纳米球，通过靶向双模式 US 成像/MRI 来识别动脉粥样硬化斑块中的巨噬细胞富集
DOI：
10.1039/c8nr04703k
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发表时间：
2018-11-21
期刊：
影响因子：
6.7
通讯作者：
Zhan, Weiwei
中科院分区：
文献类型：
--
作者：
Ji, Ri;Li, Xiaoyu;Zhan, Weiwei
巨噬细胞募集已成为驱动动脉粥样硬化病变发生和发展的关键力量。因此，斑块中巨噬细胞的识别对于易损斑块的识别至关重要，非侵入性成像方法尤为可取。有研究报道，MRI可以通过靶向纳米颗粒检测斑块中的巨噬细胞，但超声仍难以检测到动脉粥样硬化斑块中的巨噬细胞。本研究制备了靶向抗cd68受体的掺铁中空二氧化硅纳米颗粒（CD68-Fe-HSNs）作为US/MRI双峰对比剂，用于鉴定ApoE-/-小鼠腹主动脉动脉粥样硬化斑块的巨噬细胞，并通过免疫荧光和生物透射电镜证实。该体系虽然是无机介孔纳米体系，但具有可生物降解的特性，表明其具有较高的生物相容性，可用于进一步的体内研究。我们期望在未来的研究中，这些US/MRI双模态纳米颗粒将在评估易损斑块方面发挥作用。
Macrophage recruitment has emerged as the crucial force driving the initiation and progression of atherosclerotic lesions. Therefore, the identification of macrophages in plaques is of vital importance for identifying vulnerable plaques, and noninvasive imaging methods are particularly desirable. Some studies have reported that MRI can detect plaque macrophages through targeted nanoparticles, but it is still hard for an US to detect macrophages in atherosclerotic plaque. In this study, anti-CD68 receptor-targeted Fe-doped hollow silica nanoparticles (CD68-Fe-HSNs) were fabricated as a dual-modal US/MRI contrast agent for identifying macrophages of aorta ventralis atherosclerotic plaques in ApoE-/- mice, confirmed by immunofluorescence and bio-TEM. This system possesses biodegradable characteristics even though it is an inorganic mesoporous nanosystem, indicating its potential high biocompatibility for further in vivo research. We expect that these dual-modal US/MRI nanoparticles will play a role in assessing vulnerable plaque in future research studies.