Protein discrimination based on DNA induced perylene probe self-assembly
Protein discrimination based on DNA induced perylene probe self-assembly
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基于 DNA 诱导苝探针自组装的蛋白质辨别
DOI：
10.1016/j.talanta.2020.121897
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发表时间：
2021
期刊：
影响因子：
6.1
通讯作者：
Cong Yu
中科院分区：
文献类型：
--
作者：
Wei Zhou;Jiaze Hou;Yongxin Li;Huipeng Zhou;Hui Huang;Ling Zhang;Muhammad Azhar Hayat Nawaz;Cong Yu
开发一种简单有效的高灵敏度和选择性的蛋白质鉴别方法是一个非常有趣的课题。在此，我们报告了一种新的荧光检测方法的基础上，对多种蛋白质的高效率的歧视的二萘嵌苯探针的建设。单链DNA（ssDNA）可诱导苝探针聚集，导致探针荧光猝灭。加入蛋白质后，蛋白质可以与ssDNA-探针组装体复合物相互作用，产生“开启”或进一步“关闭”荧光反应。基于上述现象设计了一种传感器阵列，该阵列能够成功实现蛋白质的识别，交叉验证准确率为100%。成功识别了9个代表性蛋白。此外，观察到蛋白质可以在不同pH的测定缓冲液中诱导对DNA-探针组装体的特征效应。因此，不同的蛋白质对具有不同pH值的测定缓冲液显示出独特的荧光响应。然后将测定缓冲液pH用作感测通道。基于线性判别分析（LDA），九种蛋白质在纳摩尔浓度下被成功区分，交叉验证的准确度为100%。此外，传感器阵列还证明了九种蛋白质的差异，而不管它们的浓度如何。所开发的传感器阵列还可以在相当低的浓度下以很高的精度检测人尿样中的蛋白质，这表明其在生物流体分析中的实用性。
The development of a simple and effective method for the highly sensitive and selective discrimination of proteins is a subject of enormous interest. Herein, we report the construction of a novel fluorescence detection method based on a perylene probe for the highly efficient discrimination of multiple proteins. Single-stranded DNA (ssDNA) could induce aggregation of the perylene probe which caused quenching of probe fluorescence. After the addition of a protein, the protein could interact with the ssDNA-probe assembly complex with “turn-on” or further “turn-off” fluorescence response. A sensor array was designed based on the above phenomena which could realize the successful discrimination of proteins with 100% accuracy of cross validation. Nine representative proteins were successfully recognized. Moreover, it was observed that a protein could induce characteristic effect on the DNA-probe assembly with varying pH of assay buffer. Thus, different proteins showed unique fluorescence response towards assay buffers having different pH values. The assay buffer pH was then utilized as a sensing channel. Based on Linear Discriminant Analysis (LDA) nine proteins were successfully discriminated at the nanomolar concentration with 100% accuracy of cross validation. Furthermore, the sensor array also demonstrated differentiation of the nine proteins regardless of their concentration. The developed sensor array could also detect the proteins with great precision in human urine sample at a quite low concentration, which suggests its practical applicability for analysis of biological fluids.