Vesnarinone inhibits angiogenesis and tumorigenicity of human oral squamous cell carcinoma cells by suppressing the expression of vascular endothelial growth factor and interleukin-8.
Vesnarinone inhibits angiogenesis and tumorigenicity of human oral squamous cell carcinoma cells by suppressing the expression of vascular endothelial growth factor and interleukin-8.
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发表时间：
2005
影响因子：
5.2
通讯作者：
K. Harada;Supriatno;H. Yoshida;Mitsunobu Sato
中科院分区：
文献类型：
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作者：
K. Harada;Supriatno;H. Yoshida;Mitsunobu Sato
维司力农是一种合成的口服正性肌力药，在体外和体内对哺乳动物细胞具有多种生物活性。已报道该药物具有抗肿瘤作用和诱导肿瘤细胞增殖活性。在本研究中，我们确定维司力农是否可以在体外和体内抑制人口腔鳞状细胞癌细胞的血管生成和生长。维司力农显著抑制体外和体内两种主要促血管生成分子血管内皮生长因子（VEGF）和白细胞介素-8（IL-8）在培养细胞和植入裸鼠皮下组织的细胞中的表达。此外，维司力农抑制核因子-κ B（NF-κ B）在体外人口腔鳞状细胞癌细胞的活性。VEGF和IL-8表达的降低与体内致瘤性降低和病变血管化减少相关。这些结果表明，维司力农可以抑制血管生成和口腔鳞状细胞癌细胞的生长通过抑制VEGF和IL-8参与阻断NF-κ B活性的表达。
Vesnarinone is a synthesized positive oral inotropic agent that has multiple biological activities on mammalian cells both in vitro and in vivo. This agent has been reported in relation to its antitumor effect with apoptosis-inducing activity. In the present study, we determined whether vesnarinone could suppress angiogenesis and growth of human oral squamous cell carcinoma cells in vitro and in vivo. Vesnarinone significantly inhibited the in vitro and in vivo expression of two major proangiogenic molecules, vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8), in cultured cells and in cells implanted into the subcutaneous tissue of nude mice. Also, vesnarinone inhibited the nuclear factor-kappa B (NF-kappaB) activity in human oral squamous cell carcinoma cells in vitro. The decreased expression of VEGF and IL-8 correlated with decreased tumorigenicity and decreased vascularization of lesions in vivo. These findings suggest that vesnarinone can suppress the angiogenesis and growth of oral squamous cell carcinoma cells by inhibiting the expression of VEGF and IL-8 involved in blockade of NF-kappaB activity.