Elevation of serum hepatocyte growth factor during granulocyte colony-stimulating factor-induced peripheral blood stem cell mobilization
Elevation of serum hepatocyte growth factor during granulocyte colony-stimulating factor-induced peripheral blood stem cell mobilization
复制标题
DOI：
10.1046/j.1365-2141.2003.04745.x
复制
发表时间：
2004-01-01
影响因子：
6.5
通讯作者：
Tanimoto, M
中科院分区：
文献类型：
--
作者：
Fujii, K;Ishimaru, F;Tanimoto, M
我们检测了异基因外周血干细胞（PBSC）移植的正常供体的血管生成因子，血管内皮生长因子（VEGF），碱性成纤维细胞生长因子（bFGF）和肝细胞生长因子（HGF）的血清水平。对23名供体给予粒细胞集落刺激因子（G-CSF）（非格司亭400 μ g/m2/d），持续5天，并在第4天和第5天进行单采。虽然bFGF在G-CSF治疗后保持在相似的水平，但血清VEGF和HGF水平分别增加了1.5倍（n = 13; P = 0.02）和6.8倍（n = 23; P < 0.0001）。第1天G-CSF给药前血清HGF水平与动员的CD 34（+）细胞数呈负相关。HGF的时程动力学显示，G-CSF给药后第二天（第2天），血清HGF水平升高至3678 pg/ml。对于自体外周血干细胞动员联合化疗和G-CSF 200 μ g/m2/d（n = 8），我们观察到类似的HGF升高，这似乎是剂量依赖性的G-CSF管理。
We examined serum levels of the angiogenic factors, vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and hepatocyte growth factor (HGF), in normal donors for allogeneic peripheral blood stem cell (PBSC) transplantation. Granulocyte colony-stimulating factor (G-CSF) (filgrastim 400 mug/m(2)/d) was administered to 23 donors for 5 d and aphereses were performed on days 4 and 5. Although bFGF remained at similar levels after G-CSF treatment, serum VEGF and HGF levels increased 1.5-fold (n = 13; P = 0.02) and 6.8-fold (n = 23; P < 0.0001) respectively. The serum HGF level before G-CSF administration on day 1 correlated inversely with mobilized CD34(+) cell numbers. Time course kinetics of HGF showed that on the day after G-CSF administration (day 2), serum HGF levels increased to 3678 pg/ml. For auto PBSC mobilization with chemotherapy and G-CSF 200 mug/m(2)/d (n = 8), we observed similar HGF elevation, which appeared to be dose-dependent on the G-CSF administered.