Bacterial Endosymbiont Localization in Hyalesthes obsoletus, the Insect Vector of Bois Noir in Vitis vinifera
Bacterial Endosymbiont Localization in Hyalesthes obsoletus, the Insect Vector of Bois Noir in Vitis vinifera
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DOI：
10.1128/aem.02121-10
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发表时间：
2011-02-01
影响因子：
4.4
通讯作者：
Daffonchio, Daniele
中科院分区：
文献类型：
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作者：
Gonella, Elena;Negri, Ilaria;Daffonchio, Daniele
欧洲葡萄藤的一种新兴疾病是Bois noir（BN），这是一种由“茄病植原体（Phytoplasma solani）”引起的植原体病，并由稻飞虱Hyalesthes obsoletus（半翅目：Cixiidae）在葡萄园中传播。在这里，我们提出了第一个完整的表征细菌群落的这种重要的疾病载体收集从BN污染的地区在皮埃蒙特，意大利。针对16 S rRNA基因的长度异质性PCR和变性梯度凝胶电泳分析揭示了与昆虫载体稳定相关的许多细菌的存在。特别是，通过PCR检测到的共生细菌在成年个体中具有高感染率，属于拟杆菌门中的“Mesidatus Sulcia muelleri”簇和γ-变形菌门中的“Mesidatus Purcelliella pentastirinorum”组，这两种细菌以前在不同的叶蝉和稻飞虱中鉴定。一个高的感染率（81%），也显示了另一种共生体属于β-变形菌，指定的HO 1-V共生体。由于16 S rRNA基因的低水平的同一性（80%），最近的亲戚，蜱Haemaphysalis longicornis的一个未表征的共生体，我们提出了新的名称“Vidania fulgoroideae。“在H. obsoletus与细胞内细菌Wolbachia pipientis、立克次氏体sp.，和“红菱藻”。“荧光原位杂交结合共聚焦激光扫描显微镜和透射电子显微镜显示，这些细菌位于肠道，睾丸和卵母细胞中。作为“Ca.“沟”通常与其他共生细菌有关，我们建议在H。obsoletus，它可能会发生在一个双方甚至三方之间的关系“钙。Sulcia”和“Ca. Purcelliella，”“Ca.维达尼亚”，或者两者都有。
One emerging disease of grapevine in Europe is Bois noir (BN), a phytoplasmosis caused by "Candidatus Phytoplasma solani" and spread in vineyards by the planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae). Here we present the first full characterization of the bacterial community of this important disease vector collected from BN-contaminated areas in Piedmont, Italy. Length heterogeneity PCR and denaturing gradient gel electrophoresis analysis targeting the 16S rRNA gene revealed the presence of a number of bacteria stably associated with the insect vector. In particular, symbiotic bacteria detected by PCR with high infection rates in adult individuals fell within the "Candidatus Sulcia muelleri" cluster in the Bacteroidetes and in the "Candidatus Purcelliella pentastirinorum" group in the Gammaproteobacteria, both previously identified in different leafhoppers and planthoppers. A high infection rate (81%) was also shown for another symbiont belonging to the Betaproteobacteria, designated the HO1-V symbiont. Because of the low level of 16S rRNA gene identity (80%) with the closest relative, an uncharacterized symbiont of the tick Haemaphysalis longicornis, we propose the new name "Candidatus Vidania fulgoroideae." Other bacterial endosymbionts identified in H. obsoletus were related to the intracellular bacteria Wolbachia pipientis, Rickettsia sp., and "Candidatus Cardinium hertigii." Fluorescent in situ hybridization coupled with confocal laser scanning microscopy and transmission electron microscopy showed that these bacteria are localized in the gut, testicles, and oocytes. As "Ca. Sulcia" is usually reported in association with other symbiotic bacteria, we propose that in H. obsoletus, it may occur in a bipartite or even tripartite relationship between "Ca. Sulcia" and "Ca. Purcelliella," "Ca. Vidania," or both.