CD4+CXCR5+PD-1+ T Follicular Helper Cells Play a Pivotal Role in the Development of Rheumatoid Arthritis
CD4+CXCR5+PD-1+ T Follicular Helper Cells Play a Pivotal Role in the Development of Rheumatoid Arthritis
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CD4( )CXCR5( )PD-1( ) T 滤泡辅助细胞在类风湿关节炎的发展中发挥关键作用
DOI：
10.12659/msm.914868
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发表时间：
2019-04-25
影响因子：
3.1
通讯作者：
Zhang, Yanli
中科院分区：
文献类型：
--
作者：
Cao, Gan;Chi, Shuhong;Zhang, Yanli
背景资料：滤泡辅助性T细胞（Tfh）是次级淋巴器官生殖中心的活化CD 4（+）T细胞亚群，在许多慢性自身免疫性炎症性疾病的发生发展中起重要作用。本研究的目的是探讨循环中Tfh细胞是否有助于类风湿关节炎（RA）的发生。材料/方法：30例符合美国风湿学会（American College of Rheology-ology）制定的诊断标准的RA患者和30例健康对照者。采用流式细胞术分析患者和DBA/1 J小鼠胶原诱导性关节炎（CIA）中Tfh细胞的频率。采用酶联免疫吸附法（ELISA）检测血清IL-21水平。结果：RA患者外周血中CD 4（+）PD-1（+）CXCR 5（+）Tfh细胞数量明显高于正常对照组，DBA/1 J小鼠CIA中CD 4（+）PD-1（+）CXCR 5（+）Tfh细胞数量明显高于正常对照组。与健康对照组相比，RA患者中Bcl-6 mRNA表达水平较高，Blimp-1 mRNA表达水平较低，IL-21表达水平在RA患者中较高，这在CIA小鼠中也观察到。此外，CIA小鼠脾脏CD 4（+）ICOS（+）CXCR 5（+）Tfh细胞的出现率明显高于对照组。CD 4（+）PD-1（+）CXCR 5（+）Tfh细胞百分率与血沉（ESR）值呈正相关（r=0.968，P
Background: T follicular helper (Tfh) cells are a subgroup of activated CD4(+) T cells in the germinal centers of secondary lymphoid organs, they play critical roles in the development of many chronic autoimmune inflammatory diseases. The aim of this study was to investigate whether circulating Tfh cells contribute to the development of rheumatoid arthritis (RA).Material/Methods: Thirty patients fulfilled the diagnosis criteria that was established by the American College of Rheumatology and 30 healthy controls were recruited. The frequency of Tfh cells in patients and collagen-induced arthritis (CIA) in DBA/1J mice were analyzed by flow cytometry. The serum IL-21 level was examined by enzyme-linked immunosorbent assay (ELISA). The mRNA expression of Blimp-1 and Bcl-6 were detected by qRT-PCR.Results: RA patients had more CD4(+)PD-1(+)CXCR5(+) Tfh cells in peripheral blood compared with healthy controls, and CIA in DBA/1J mice showed similar results. Higher mRNA expression of Bcl-6 and lower Blimp-1 mRNA expression were observed in patients with RA compared to healthy controls, and the expression level of IL-21 was higher in RA patients, which was also seen in CIA mice. Furthermore, the spleen CD4(+)ICOS(+)CXCR5(+) Tfh cells in CIA mice show significantly higher frequency than that in the control mice. The percentage of CD4(+)PD-1(+)CXCR5(+) Tfh cells was correlated positively with the values of erythrocyte sedimentation rate (ESR) (r=0.968, P