Mitosis-Mediated Intravasation in a Tissue-Engineered Tumor-Microvessel Platform.
Mitosis-Mediated Intravasation in a Tissue-Engineered Tumor-Microvessel Platform.
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DOI：
10.1158/0008-5472.can-16-3279
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发表时间：
2017-11-15
期刊：
影响因子：
11.2
通讯作者：
Searson PC
中科院分区：
文献类型：
--
作者：
Wong AD;Searson PC
Intravasation involves the migration of tumor cells across the local endothelium and escape into vessel flow. While tumor cell invasiveness has been correlated to increased intravasation, the details of transendothelial migration and detachment into circulation are still unclear. Here we analyzed the intravasation of invasive human breast cancer cells within a tissue-engineered microvessel model of the tumor microenvironment. Using live-cell fluorescence microscopy, we captured 2,330 hours of tumor cell interactions with functional microvessels and provide evidence for a mitosis-mediated mechanism where tumor cells located along the vessel periphery are able to disrupt the vessel endothelium through cell division and detach into circulation. This model provides a framework for understanding the physical and biological parameters of the tumor microenvironment that mediate intravasation of tumor cells across an intact endothelium.
