Entire thickness profiles of the epithelium and contact lens in vivo imaged with high-speed and high-resolution optical coherence tomography.
Entire thickness profiles of the epithelium and contact lens in vivo imaged with high-speed and high-resolution optical coherence tomography.
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DOI：
10.1097/icl.0b013e31829fae00
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发表时间：
2013-09
期刊：
影响因子：
--
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Tao A;Shao Y;Jiang H;Ye Y;Lu F;Shen M;Zhu D;Wang J
测试使用高速和高分辨率的谱域光学相干断层扫描（SD-OCT）在体内测量上皮和接触透镜的整个厚度轮廓的可行性。基于CMOS相机开发了定制的长扫描深度SD-OCT，组织中的轴向分辨率约为5.1 µm。5名受试者的5只眼睛在佩戴一个接触透镜（CL）之前和佩戴时跨水平子午线成像两次。进行半自动测量以在校正光学畸变后产生上皮、总角膜和接触透镜的整个厚度轮廓。上皮、眼表和接触透镜的全宽和全深清晰可见。中央的上皮厚度（ET）为51.9 ± 3.5 µm，在直径为7 mm的中央保持该厚度，然后在颞部和鼻周边增加。接触透镜轮廓显示中心最薄点，厚度为100.3 ± 4.9 µm。厚度朝中周边增加，然后在边缘处减小。该初步研究证明了使用基于高速CMOS的OCT在体内评价上皮和接触透镜的整个厚度轮廓的可行性。需要进一步开发，以将扫描从2D扩展到3D，并具有强大的自动图像处理能力。
To test the feasibility of measuring the entire thickness profiles of the epithelium and contact lens in vivo, using high speed and high resolution spectral domain optical coherence tomography (SD-OCT). A custom-built, long scan depth SD-OCT was developed based on a CMOS camera and the axial resolution was about 5.1 µm in tissue. Five eyes of 5 subjects were imaged twice across the horizontal meridian before and while wearing one contact lens (CL). Semi-automatic measurement was done to yield the entire thickness profiles of the epithelium, total cornea, and contact lens after correcting for optical distortion. The full width and depth of the epithelium, ocular surface and contact lens were clearly visualized. The epithelial thickness (ET) at the center was 51.9 ± 3.5 µm, it remained at this thickness across the central 7 mm diameter and then increased at both temporal and nasal peripheries. The contact lens profile showed the thinnest point at the center with thickness of 100.3 ± 4.9 µm. The thickness increased towards the mid-periphery and then decreased at the edge. This pilot study demonstrated the feasibility of using high speed CMOS-based OCT to evaluate the entire thickness profiles of the epithelium and contact lens in vivo. Further development will be needed to extend the scanning from 2D to 3D with a robust automatic image processing ability.