PHOSPHOLIPASE-INDUCED MATURATION OF XENOPUS-LAEVIS OOCYTES - MITOGENIC ACTIVITY OF GENERATED METABOLITES
PHOSPHOLIPASE-INDUCED MATURATION OF XENOPUS-LAEVIS OOCYTES - MITOGENIC ACTIVITY OF GENERATED METABOLITES
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DOI：
10.1002/jcb.240520408
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发表时间：
1993-08-01
影响因子：
4
通讯作者：
LACAL, JC
中科院分区：
文献类型：
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作者：
CARNERO, A;LACAL, JC
由膜磷脂产生的第二信使诱导的信号转导被认为是控制细胞增殖的主要调节机制。我们在这里报告，在非洲爪蟾卵母细胞模型中，显微注射的三个最相关类型的磷脂酶作用于膜磷脂（A2，C和D）能够诱导卵母细胞成熟与类似的效率。这种作用是由已知的第二信使如溶血磷脂，花生四烯酸，甘油二酯和磷脂酸的产生介导的。蛋白激酶C的特异性抑制剂使得有可能识别用于诱导卵母细胞成熟的替代性独立信号传导途径。我们的研究结果表明，虽然磷脂酶C似乎是依赖于蛋白激酶C（PKC），磷脂酶A2和磷脂酶D是完全独立的蛋白激酶C功能。因此，卵母细胞系统是分析脂质代谢物潜在促有丝分裂活性的有力工具。它也是一个很好的工具，用于解开参与细胞生长调节的不同途径。 (c)1993 Wiley-Liss，Inc.
Signal transduction induced by generation of second messengers from membrane phopholipids is considered a major regulatory mechanism in control of cell proliferation. We report here that in the Xenopus laevis oocytes model, microinjection of the three most relevant types of phospholipases acting on membrane phospholipids (A2, C, and D) are capable of inducing oocyte maturation with similar efficiencies. This effect is mediated by the generation of known second messengers such as lyso-phospholipids, arachidonic acid, diacylglycerol, and phosphatidic acid. Specific inhibitors of protein kinase C made it possible to identify alternative independent signalling pathways for induction of oocyte maturation. Our results indicate that while phospholipase C seems to be dependent on protein kinase C (PKC), phospholipase A2, and phospholipase D are completely independent of protein kinase C function. Thus, the oocyte system is a powerful tool for the analysis of the potential mitogenic activity of lipid metabolites. It is also an excellent tool for unravelling the different routes involved in the regulation of cell growth. (c) 1993 Wiley-Liss, Inc.