Genomic relationships among sixteen species of Avena based on (ACT)(6) trinucleotide repeat FISH
Genomic relationships among sixteen species of Avena based on (ACT)(6) trinucleotide repeat FISH
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基于 (ACT)(6) 三核苷酸重复 FISH 的十六种燕麦之间的基因组关系
DOI：
10.1139/gen-2017-0132
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发表时间：
2018
期刊：
影响因子：
3.1
通讯作者：
Peng Yuanying
中科院分区：
文献类型：
--
作者：
Luo Xiaomei;Liu Juncheng;Wan Wenlin;Chen Liang;Tinker Nick A.;Wight Charlene P.;Zhou Yonghong;Peng Yuanying
了解重复元件的位置对基因组序列的组装及其对物理染色体的分配是非常重要的。利用荧光原位杂交技术（FISH）和Am1和（ACT）6探针对16个物种的基因组和物种关系进行了研究。Am1寡核苷酸探针在C基因组中富集，而（ACT）6三核苷酸重复探针在a、AB、C、AC和ACD基因组中显示出多样化的杂交模式分布，但在B和D基因组中可能不存在。玉米的杂交模式与玉米的杂交模式非常相似。这表明这个物种很可能起源于马。岛属四倍体祖先。尽管两种FISH探针未能识别更多物种的关系，但这种概念验证方法为使用FISH探针将基因组序列中的其他特征元素分配给物理染色体开辟了道路。
Knowledge of the locations of repeat elements could be very important in the assembly of genome sequences and their assignment to physical chromosomes. Genomic and species relationships among 16 species were investigated using fluorescence in situ hybridization (FISH) with the Am1 and (ACT)6probes. The Am1 oligonucleotide probe was particularly enriched in the C genomes, whereas the (ACT)6trinucleotide repeat probe showed a diverse distribution of hybridization patterns in the A, AB, C, AC, and ACD genomes but might not be present in the B and D genomes. The hybridization pattern ofAvena sativawas very similar to that ofA. insularis, indicating that this species most likely originated fromA. insularisas a tetraploid ancestor. Although the two FISH probes failed to identify relationships of more species, this proof-of-concept approach opens the way to the use of FISH probes in assigning other signature elements from genomic sequence to physical chromosomes.