EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells.
EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells.
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EphA2增强LNCaP前列腺癌细胞的增殖和侵袭能力
DOI：
10.3892/ol.2014.2093
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发表时间：
2014-07
期刊：
影响因子：
2.9
通讯作者：
Bai P
中科院分区：
文献类型：
--
作者：
Chen P;Huang Y;Zhang B;Wang Q;Bai P
EphA2在许多人类癌症中持续过表达和功能改变。然而，据我们所知，EphA2在前列腺癌中所起的作用并不完全清楚。为了探讨EphA2在前列腺癌发生发展中的作用，本研究采用重组质粒、western blot分析、流式细胞术、Matrigel侵袭室和细胞计数试剂盒-8等方法初步评价了EphA2蛋白在LNCaP前列腺癌细胞中的作用。通过免疫组化方法观察EphA2在前列腺癌组织中的作用。结果表明，转染pcDNA3.1（+）质粒介导的pcDNA3.1(+)-EphA2后，LNCaP人前列腺癌细胞的体外生长和侵袭能力明显增强。EphA2在前列腺癌标本中过表达，且EphA2的表达与Gleason分级、前列腺总特异性抗原、临床分期及淋巴结转移显著相关。总之，这些结果表明EphA2参与恶性细胞行为，是人类前列腺癌的潜在治疗靶点。
EphA2 is persistently overexpressed and functionally changed in numerous human cancers. However, to the best of our knowledge, the role that EphA2 plays in prostate cancer is not entirely clear. To investigate the roles of EphA2 in the development and progression of prostate cancer, the present study initially evaluated the roles of the EphA2 protein in LNCaP prostate cancer cells using recombinant plasmid, western blot analysis, flow cytometry, Matrigel invasion chamber and the cell counting kit-8 assay. An immunohistochemistry assay was also conducted to observe the effects of EphA2 in prostate cancer tissues. The results demonstrated that the LNCaP human prostate cancer cells that were transfected with pcDNA3.1(+) plasmid-mediated pcDNA3.1(+)-EphA2, markedly enhanced the cell growth and invasion in vitro. Additionally, EphA2 was overexpressed in prostate cancer specimens and the expression of EphA2 was significantly associated with Gleason grade, total prostate-specific antigen, advanced clinical stage and lymph node metastasis. Collectively, these results demonstrate that EphA2 is involved in malignant cell behavior and is a potential therapeutic target in human prostate cancer.
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