Biodegradation of deoxynivalenol and its derivatives by Devosia insulae A16
Biodegradation of deoxynivalenol and its derivatives by Devosia insulae A16
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Devosia insulae A16 生物降解脱氧雪腐镰刀菌烯醇及其衍生物
DOI：
10.1016/j.foodchem.2018.10.011
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发表时间：
2019-03-15
期刊：
影响因子：
8.8
通讯作者：
Xu, Jianhong
中科院分区：
文献类型：
--
作者：
Wang, Gang;Wang, Yanxia;Xu, Jianhong
脱氧雪腐镰刀菌烯醇(DON)是一种臭名昭著的真菌毒素，主要存在于受镰刀菌污染的农作物中，给畜牧业造成巨大损失，并对人类健康构成严重的安全威胁。本文报道了一株对DON及其衍生物3-乙酰基-DON和15-乙酰基-DON具有有效生物降解能力的革兰氏阴性菌的分离。根据形态和生理特性以及16S rRNA的系统发育分析，该菌株被鉴定为Devosia sulae A16。在35℃、中性pH的好氧条件下，胰岛素假单胞菌A16能在48h内降解20 mg/L DON，降解率达88%。DON及其衍生物的主要降解产物为3-酮-DON，其主要降解产物为C-3上的羟基。3-乙酰基-DON和15-乙酰基-DON均经过脱乙酰基反应生成DON，然后降解为3-酮基-DON。这些结果为海藻A16作为生物降解剂控制谷物中DON污染提供了潜在的用途。
Deoxynivalenol (DON), a notorious mycotoxin mainly found in Fusarium-contaminated crops, causes great loss in livestock farming and severe safety risks to human health. Here we report the isolation of a Gram-negative bacterial strain with effective biodegrading abilities on DON and its derivatives including 3-acetyl-DON and 15-acetyl-DON. The strain was identified as Devosia insulae A16 on the basis of morphological and physiological characteristics and 16S rRNA-based phylogenetic analysis. D. insulae A16 was able to degrade 88% of 20 mg/l DON within 48 h under aerobic conditions at 35 degrees C and neutral pH. The major degradation product of DON and its derivatives was 3-keto-DON by the oxidation of the hydroxyl group at C-3. Both 3-acetyl-DON and 15-acetyl-DON underwent a deacetylation reaction to generate DON prior to the degradation to 3-keto-DON. The results provide the potential use of D. insulae A16 as a biodegradation agent to control DON contamination in cereals.