The interactions between the fluorescent dye thiazole orange and DNA.
The interactions between the fluorescent dye thiazole orange and DNA.
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DOI：
10.1002/(sici)1097-0282(199807)46:1
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发表时间：
1998-07
期刊：
影响因子：
2.9
通讯作者：
J. Nygren;Nicke Svanvik;M. Kubista
中科院分区：
文献类型：
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作者：
J. Nygren;Nicke Svanvik;M. Kubista
研究了荧光染料噻唑橙子（TO）与核酸的相互作用。发现TO以最高亲和力结合双链（ds）DNA [log（K）在100 mM盐下约为5.5]，比单链聚嘌呤弱约5-10倍，并且比单链聚嘧啶弱10-1000倍。TO作为单体结合至dsDNA和聚（dA），作为单体和作为二聚体结合至聚（dG），并且主要作为二聚体结合至聚（dC）和聚（dT）。溶液中游离TO的荧光量子产率为约2 × 10 - 4，当与dsDNA或poly（dA）结合时增加至约0.1，当与poly（dG）结合时增加至约0.4。结合到聚（dC）和聚（dT）的TO的估计量子产率分别为约0.06和0.01。结合TO的量子产率取决于温度，并且在5至50摄氏度之间降低约三倍。
The interaction of the fluorescent dye thiazole orange (TO) with nucleic acids is characterized. It is found that TO binds with highest affinity to double-stranded (ds) DNA [log (K) approximately 5.5 at 100 mM salt], about 5-10 times weaker to single-stranded polypurines, and further 10-1000 times weaker to single-stranded polypyrimidines. TO binds as a monomer to dsDNAs and poly(dA), both as a monomer and as a dimer to poly(dG) and mainly as a dimer to poly(dC) and poly(dT). The fluorescence quantum yield of TO free in solution is about 2 x 10(-4), and it increases to about 0.1 when bound to dsDNA or to poly(dA), and to about 0.4 when bound to poly(dG). Estimated quantum yields of TO bound to poly(dC) and poly(dT) are about 0.06 and 0.01, respectively. The quantum yield of bound TO depends on temperature and decreases about threefold between 5 and 50 degrees C.