Tyrosine Phosphorylation of the 2B Subunit of the NMDA Receptor Is Necessary for Taste Memory Formation
Tyrosine Phosphorylation of the 2B Subunit of the NMDA Receptor Is Necessary for Taste Memory Formation
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DOI：
10.1523/jneurosci.5667-08.2009
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发表时间：
2009-07-22
影响因子：
5.3
通讯作者：
Rosenblum, Kobi
中科院分区：
文献类型：
--
作者：
Barki-Harrington, Liza;Elkobi, Alina;Rosenblum, Kobi
我们的目的是测试是否在岛叶皮层的NMDA受体（NMDAR）的酪氨酸磷酸化是必要的新的味觉学习。我们发现，在大鼠中，新的味觉学习导致NMDAR的NR 2B亚基的酪氨酸1472磷酸化升高，并增加磷酸化NR 2B与主要突触后支架蛋白PSD-95的相互作用。注射酪氨酸激酶抑制剂染料木黄酮直接进入岛皮质的大鼠之前，新的味道曝光防止增加NR 2B酪氨酸磷酸化和行为衰减味觉记忆的形成。在功能上，NR 2B的酪氨酸磷酸化学习后被发现，以确定突触分布的NMDAR，因为微量注射染料木素的岛叶皮质改变了NMDAR的分布模式所造成的新的味觉学习。
We aimed to test whether tyrosine phosphorylation of the NMDA receptor (NMDAR) in the insular cortex is necessary for novel taste learning. We found that in rats, novel taste learning leads to elevated phosphorylation of tyrosine 1472 of the NR2B subunit of the NMDAR and increases the interaction of phosphorylated NR2B with the major postsynaptic scaffold protein PSD-95. Injection of the tyrosine kinase inhibitor genistein directly into the insular cortex of rats before novel taste exposure prevented the increase in NR2B tyrosine phosphorylation and behaviorally attenuated taste-memory formation. Functionally, tyrosine phosphorylation of NR2B after learning was found to determine the synaptic distribution of the NMDAR, since microinjection of genistein to the insular cortex altered the distribution pattern of NMDAR caused by novel taste learning.