Sperm DNA fragmentation in infertile men with genitourinary infection by Chlamydia trachomatis and Mycoplasma
Sperm DNA fragmentation in infertile men with genitourinary infection by Chlamydia trachomatis and Mycoplasma
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DOI：
10.1016/j.fertnstert.2007.06.035
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发表时间：
2008-08-01
影响因子：
6.7
通讯作者：
Luis Fernandez, Jose
中科院分区：
文献类型：
--
作者：
Gallegos, Guadalupe;Ramos, Benito;Luis Fernandez, Jose
目的：采用Halosperm试剂盒进行精子染色质分散试验，确定泌尿生殖道沙眼衣原体和支原体感染男性精液样本中DNA片段化精子细胞的频率以及抗生素治疗的影响。设计：前瞻性研究。地点：大学附属生殖医学中心、医学遗传学实验室和学术生物学中心。患者：143名男性成员的夫妇参加了男科不孕不育中心和一组50名生育受试者。对95例男性患者进行了抗生素治疗效果评价。干预措施：无。主要结果测量：经典精液分析（浓度、活力、形态和活力）、精子DNA片段化和临床结果。精子DNA断裂率为35.2%~ 13.5%，是对照组（10.8%~ 5.6%）的3.2倍。浓度、形态和运动性也受到显著影响，但程度要低得多。精子活力没有受到显著影响。抗生素治疗3.8 ~ 2.2个月后，精子DNA片段化率由治疗前的37.7%~ 13.6%下降到24.2%~ 11.2%。精子浓度和活力没有明显改善。在一组16对在抗生素治疗过程中试图怀孕的夫妇中，只有12.5%的人成功怀孕。然而，在一组的14对夫妇谁试图怀孕后完成抗生素治疗，85.7%实现it. The唯一显着差异组间发现的是精子DNA片段化率和morphology. Conclusions（s）：患者生殖道沙眼衣原体和支原体感染增加精子DNA片段化与生育对照组相比。这种增加在比例上大于对经典精液参数的影响，并可能导致生育潜力降低。抗生素治疗似乎是重要的感染诱导的高DNA片段化水平的补救措施。
Objective: To determine the frequency of sperm cells with fragmented DNA in semen samples from men with goenitourinary infection by Chlainydia trachomatis and Mycoplasina and the influence of antibiotic therapy, using the sperm chromatin dispersion test with the Halosperm, kit.Design: Prospective study.Setting: University-affiliated reproductive medicine center, medical genetics laboratory, and academic biology center.Patient(s): One hundred forty-three male member of couples attending the andrology infertility center and a group of 50 fertile subjects. The effect of antibiotic treatment was evaluated in 95 male patients.Intervention(s): None.Main Outcome Measure(s): Classical semen analysis (concentration, motility, morphology, and vitality), sperm DNA fragmentation, and clinical outcome.Result(s): The mean percentage of sperm cells with fragmented DNA was 35.2% 13.5%, 3.2 times higher than in the control fertile group (10.8% 5.6%). Concentration, morphology, and motility were also significantly affected but to a much lower degree. Sperm vitality was not significantly affected. After 3.8 2.2 months of antibiotic treatment, the mean frequency of spermatozoa with fragmented DNA decreased from 37.7% 13.6% to 24.2% 11.2%. Sperm concentration and motility were not significantly improved. In a group of 16 couples who attempted pregnancy during antibiotic treatment course, only 12.5% achieved pregnancy. However, in a group of 14 couples who attempted pregnancy after finishing the antibiotic treatment, 85.7% achieved it. The only significant differences found between groups was the rate of sperm DNA fragmentation and morphology.Conclusion(s): Patients with genitourinary infection by Chlaniydia trachomatis and Mycoplasina have increased sperm DNA fragmentation in comparison with fertile controls. This increase is proportionally greater than the influence on classical semen parameters and could result in a decreased fertility potential. Antibiotic therapy appears to be important in providing a remedy for infection-induced high DNA fragmentation levels.