Physiological and biochemical changes associated with flower development and senescence in Dianthus chinensis L
Physiological and biochemical changes associated with flower development and senescence in Dianthus chinensis L
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DOI：
10.1007/s40502-014-0104-9
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发表时间：
2014-09-01
期刊：
影响因子：
--
通讯作者：
Ahmad, Syed Sabhi
中科院分区：
文献类型：
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作者：
Dar, Riyaz Ahmad;Tahir, Inayatullah;Ahmad, Syed Sabhi
以克什米尔大学植物园（KUBG）的石竹（Dianthus chinensis）为研究对象。花的发育和衰老分为6个阶段（I-VI），分别为(I)紧芽期、（II）成熟芽期、（III）刷漆期、（IV）完全开放/开花期、(V)部分衰老期和（VI）衰老期。记录了与花发育和衰老相关的各种生理生化变化。鲜质量、干质量、含水量和花径从花蕾到开花呈连续增加的趋势，即从第I期到第IV期，从第V期到第VI期显著下降。扫描电镜研究表明，随着衰老的开始，石竹的细胞完整性和结构明显退化。可溶性蛋白、α -氨基酸和糖含量随开花而增加，随衰老而减少。花瓣组织的SDS-PAGE显示高分子量和低分子量蛋白均减少。目前的研究表明，蛋白质降解是调控花衰老过程的关键因素。
Flowers of Dianthus chinensis growing in Kashmir University Botanic Garden (KUBG) were selected for the present study. Flower development and senescence was divided into six stages (I-VI), categorized as (I) tight bud stage, (II) mature bud stage, (III) paint brush stage, (IV) fully open/bloom stage, (V) partially senescent stage and (VI) senescent stage. Various physiological and biochemical changes associated with flower development and senescence were recorded. Fresh and dry mass, water content and flower diameter showed a continuous increase from bud to bloom, i.e., from stage I IV and a significant decrease from stage V to VI. Scanning electron microscopic studies showed a clear degeneration of the cellular integrity and architecture with the onset of senescence in Dianthus chinensis. Soluble proteins, alpha-amino acids and sugar fractions increased with flower opening and showed a decrease as the senescence progressed. SDS-PAGE of the petal tissues revealed a decrease in both high and low molecular weight proteins. The present study suggests that the protein degradation is the key factor in regulating the process of flower senescence in this flower.