Scanning electrochemical microscopy of living cells. 5. Imaging of fields of normal and metastatic human breast cells.
Scanning electrochemical microscopy of living cells. 5. Imaging of fields of normal and metastatic human breast cells.
复制标题
DOI：
10.1021/ac0343127
复制
发表时间：
2003-07
影响因子：
7.4
通讯作者：
Wenju Feng;S. Rotenberg;M. Mirkin
中科院分区：
文献类型：
--
作者：
Wenju Feng;S. Rotenberg;M. Mirkin
扫描电化学显微镜（SECM）用于对单层培养中不同类型的人类乳腺细胞的视野进行成像。这些实验的目的是证明通过氧化还原活性区分未转化的人乳腺上皮细胞 (MCF-10A) 和转移性乳腺细胞 (MDA-MB-231) 的可能性。通过 SECM 对密集细胞进行成像所需的方法与之前报道的分离良好的单细胞实验不同。 SECM 与光学和荧光显微镜相结合，用于在同质或异质细胞区域中定位单个细胞。为了确定可以在正常细胞区域中检测到转移性乳腺细胞，前者预先装载了荧光纳米球，并与未标记的 MCF-10A 细胞一起铺板。通过匹配选定的一组转移细胞的 SECM 和荧光图像，可以显示 SECM 信号的辨别水平和保真度。确定了几个因素（电极和细胞之间的距离、细胞密度、介质的选择及其浓度），这些因素可用于最大化转移细胞和非转化细胞图像之间的对比度。这些研究为未来分析人类乳腺组织样本中的恶性细胞提供了框架。
Scanning electrochemical microscopy (SECM) was used to image fields of different types of human breast cells in monolayer culture. The goal of these experiments was to demonstrate the possibility of distinguishing between nontransformed human breast epithelial cells (MCF-10A) and metastatic breast cells (MDA-MB-231) by their redox activities. Imaging of densely packed cells by SECM requires approaches that differ from previously reported experiments with well-separated single cells. The combination of SECM with optical and fluorescence microscopies was used to locate individual cells in a homogeneous or heterogeneous field of cells. To establish that metastatic breast cells can be detected against a field of normal cells, the former were preloaded with fluorescent nanospheres and plated together with unlabeled MCF-10A cells. By matching SECM and fluorescence images of a selected group of metastatic cells, the level of discrimination and fidelity of the SECM signal could be shown. Several factors (distance between the electrode and the cells, cell density, choice of mediator, and its concentration) were identified that can be used to maximize the contrast between images of metastatic and nontransformed cells. These studies provide a framework for future analysis of malignant cells in human breast tissue samples.