RT-PCR analysis of Tecta, Coch, Eya4 and Strc in mouse cochlear explants
RT-PCR analysis of Tecta, Coch, Eya4 and Strc in mouse cochlear explants
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DOI：
10.1097/00001756-200503150-00011
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发表时间：
2005-03-15
期刊：
影响因子：
1.7
通讯作者：
Smith, RJH
中科院分区：
文献类型：
--
作者：
Maeda, Y;Fukushima, K;Smith, RJH
Tecta、Coch、Eya 4和Strc是四种人类生殖相关基因的小鼠直系同源物。它们的表达明显局限于耳蜗中的特定细胞类型。在胚胎第15天解剖耳蜗并进行体外培养。每个基因的相对信使RNA丰度定量RT-PCR和比较体内耳蜗的胚胎年龄相当。培养48 h后，体内和外植体Strc表达水平相当，Eya 4水平在移植组织中降低，Tecta和Coch的表达没有显示出预期的时间上升。即使在96 h后也可检测到这些基因的表达。这些结果表明，在耳蜗发育过程中检测内耳特异性基因的表达是可行的。(c)2005年利平科特威廉姆斯&威尔金斯。
Tecta, Coch, Eya4 and Strc are mouse orthologs of four human deafness-associated genes. Their expression is markedly restricted to specific cell types in cochleae. Cochleae were dissected on embryonic day 15 and cultured in vitro. Relative messenger RNA abundance of each gene was quantified by RT-PCR and compared in-vivo cochleae of equivalent embryonic age. After 48 h in culture, in-vivo and explant Strc expression levels were equivalent, Eya4 level reduced in explanted tissues, and expression of Tecta and Coch did not show the expected temporal rise. Expression of these genes was detectable even after 96 h. These results suggest that it is feasible to test the expression of inner ear specific genes in explanted cochleae. (c) 2005 Lippincott Williams & Wilkins.