Estrogen is necessary for 5α-pregnan-3α-ol-20-one (3α,5α-THP) infusion to the ventral tegmental area to facilitate social and sexual, but neither exploratory nor affective behavior of ovariectomized rats
Estrogen is necessary for 5α-pregnan-3α-ol-20-one (3α,5α-THP) infusion to the ventral tegmental area to facilitate social and sexual, but neither exploratory nor affective behavior of ovariectomized rats
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DOI：
10.1016/j.pbb.2008.08.018
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发表时间：
2008-12-01
影响因子：
3.6
通讯作者：
Rhodes, M. E.
中科院分区：
文献类型：
--
作者：
Frye, C. A.;Paris, J. J.;Rhodes, M. E.
孕酮代谢物5 α -孕-3 α -醇-20- 1 （3 α,5 α - thp，异孕酮）在腹侧被覆区（VTA）中起作用，促进卵巢切除（OVX）、雌激素（E(2)）启动大鼠和性腺完整大鼠在高（发情）或低（退情）内源性E(2)水平下的探索、抗焦虑和社会性行为。3 α，5 α - thp的这些作用需要多少E(2)尚不清楚。OVX大鼠全身灌注17 -雌二醇（10 μ g）或油载体，44 h后向VTA、黑质（SN）或中央灰色（CC）注入3 - α、5 - α - thp （100 ng）或β -环糊精载体。研究人员对大鼠进行了一系列的评估，包括探索性（野外）、焦虑性（升高加上迷宫）、社会性（伴侣偏好、社会互动）和性（有节奏的交配）任务。E(2)-启动是3 α，5 α - thp输注促进社会交往和交配所必需的，E(2)-输注中脑3 α，5 α - thp水平高于载体启动大鼠。不考虑E(2)-启动，向VIA注入3 α，5 α - thp的大鼠，而不注入SN或CC，表现出在开放领域的探索增加，在高难度迷宫中的抗焦虑行为，以及对雄性的偏好。因此，VTA中3 α，5 α - thp增强社交和性行为的作用依赖于E(2)，而探索和抗焦虑行为的增加不依赖于E(2)。(C) 2008爱思唯尔公司版权所有。
The progesterone metabolite, 5 alpha-pregnan-3 alpha-ol-20-one (3 alpha,5 alpha-THP, allopregnanolone), acts in the ventral tegmental area (VTA) to facilitate exploratory, anti-anxiety, and socio-sexual behavior among ovariectomized (OVX), estrogen (E(2))-primed rats and gonadally-intact rats with high (proestrus) or low (diestrus) endogenous E(2) levels. The extent to which E(2) is required for these effects of 3 alpha,5 alpha-THP is not known. OVX rats were primed with systemic 17 beta-estradiol (10 mu g) or oil vehicle and were infused 44 h later with 3 alpha,5 alpha-THP (100 ng) or beta-cyclodextrin vehicle to the VTA, substantia nigra (SN), or central grey (CC). Rats were assessed in a battery of exploratory (open field), anxiety (elevated plus maze), social (partner preference, social interaction), and sexual (paced mating) tasks. E(2)-priming was necessary for 3 alpha,5 alpha-THP infusions to facilitate social interaction and mating and midbrain 3 alpha,5 alpha-THP levels were higher among E(2)-compared to vehicle-primed rats. Irrespective of E(2)-priming, rats infused with 3 alpha,5 alpha-THP to the VIA, but not SN or CC, demonstrated increased exploration in an open field, anti-anxiety behavior on an elevated plus maze, and preference for a male. Thus, actions of 3 alpha,5 alpha-THP in the VTA to enhance social and sexual behaviors were reliant on E(2) but increases in exploratory and anti-anxiety behavior were not. (C) 2008 Elsevier Inc. All rights reserved.