Ribosomal DNA inheritance and recombination in Neurospora crassa.
Ribosomal DNA inheritance and recombination in Neurospora crassa.
复制标题
粗糙脉孢菌中核糖体 DNA 的遗传和重组。
DOI：
10.1007/bf00425714
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Wagner,S
中科院分区：
文献类型：
--
作者：
Russell,PJ;Petersen,RC;Wagner,S
利用9个实验室野生型菌株和野生采集菌株的非转录间隔区(NTS)序列中的限制性片段长度多态性，分析了核糖体DNA(RDNA)在粗糙脉孢菌中的遗传分离。在对七个杂交组合的随机孢子后代和其中两个杂交组合的有序四分体的分析中，rDNA以简单、稳定的孟德尔方式遗传，表现出两种亲本rDNA类型的大约1：1的比例。在后代中没有检测到减数分裂重组子，这表明rDNA区域的非姐妹染色单体互换受到高度抑制。这种抑制减数分裂重组的基础尚不清楚。
The genetic segregation of ribosomal DNA (rDNA) inNeurospora crassawas analyzed by exploiting restriction fragment length polymorphisms in the nontranscribed spacer (NTS) sequences of nine laboratory wild-type strains and wild-collected strains. In an analysis of random spore progeny from seven crosses, and of ordered tetrads from two of those crosses the rDNA was shown to be inherited in a simple, stable Mendelian fashion, exhibiting an approximately 1:1 ratio of the two parental rDNA types. No meiotic recombinants were detected among the progeny, indicating that non-sister-chromatid crossing over is highly suppressed in the rDNA region. The basis for this suppression of meiotic recombination is not known.