Immunohistochemical detection of a hypoxia marker in spontaneous canine tumours.
Immunohistochemical detection of a hypoxia marker in spontaneous canine tumours.
复制标题
自发犬肿瘤中缺氧标记的免疫组织化学检测。
DOI：
10.1038/bjc.1990.411
复制
发表时间：
1990-12
影响因子：
8.8
通讯作者：
Raleigh, J A
中科院分区：
文献类型：
--
作者：
Cline, J M;Thrall, D E;Page, R L;Franko, A J;Raleigh, J A
免疫过氧化物酶技术已被用来检测在体内结合的2-硝基咪唑缺氧标记在各种切除犬肿瘤的组织化学切片。结合发生在距离肿瘤血管10-12个细胞直径处，这与缺氧细胞在组织中的预期位置一致，其中氧浓度梯度是通过扩散建立的。根据对多个肿瘤切片的形态测定分析，估计了4 - 13%的低分化分数。标记物的结合仅限于细胞的细胞质。标记物出现在坏死附近区域，但也出现在无坏死区域。在早期的放射自显影研究中，偶尔在肿瘤血管附近的细胞中观察到结合。通常，未观察到与正常组织的结合。然而，在正常组织和肿瘤组织的一些切片中观察到与小动脉周围的平滑肌细胞结合。
An immunoperoxidase technique has been used to detect the in vivo binding of a 2-nitroimidazole hypoxia marker in histochemical sections of a variety of excised canine tumours. The binding occurred 10-12 cell diameters away from tumour blood vessels, consistent with the expected location of hypoxic cells in tissues in which oxygen concentration gradients are established by diffusion. Hypoxic fractions ranging from 4 to 13% have been estimated on the basis of morphometric analysis of multiple tumour sections. The binding of the marker was restricted to the cytoplasm of the cells. The marker appeared in regions adjacent to necrosis but also in regions free of necrosis. As in earlier autoradiography studies, binding was occasionally observed in cells adjacent to tumour blood vessels. Generally, binding to normal tissues was not observed. However, binding to smooth muscle cells surrounding arterioles in some sections of normal tissue and tumour tissue was observed.