Expression, characterization and immunogenicity of flagellin FlaC from Vibrio alginolyticus strain HY9901
Expression, characterization and immunogenicity of flagellin FlaC from Vibrio alginolyticus strain HY9901
复制标题
溶藻弧菌 HY9901 菌株鞭毛蛋白 Flac 的表达、表征和免疫原性
DOI：
10.1016/j.fsi.2010.04.003
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发表时间：
2010-08-01
影响因子：
4.7
通讯作者：
Lu, Yishan
中科院分区：
文献类型：
--
作者：
Liang, Haiying;Xia, Liqun;Lu, Yishan
溶藻弧菌是感染人类和海洋动物的一种普遍存在的病原体。细菌的Flagellins在感染动物和诱导宿主免疫反应中发挥着重要作用。本研究克隆并表达了溶藻弧菌HY9901鞭毛蛋白FLAC基因。FLAC基因开放阅读框全长1155bp，编码384个氨基酸残基。用纯化的重组FLAC蛋白在小鼠体内制备多克隆抗体，用溶藻弧菌FLAC蛋白和粗蛋白提取物进行免疫印迹分析，证实抗体的反应性。重组FLAC免疫红鳍金枪鱼(Lutjanus Sanguineus)可产生特异性抗体，对强毒溶藻弧菌具有较强的抵抗力。本研究表明，保守的FLAC是预防溶藻弧菌感染的有效候选疫苗。(C)2010爱思唯尔有限公司。保留所有权利。
Vibrio alginolyticus is one of ubiquitous pathogens infecting human and marine animals. Flagellins of bacteria play an important role in infecting animals and inducing host immune response. In the present research, flagellin flaC gene of V. alginolyticus strain HY9901 was cloned and expressed. The open reading frame of flaC gene contains 1155 bp and the putative protein consists of 384 amino acid residues. Polyclonal antibodies were raised in mouse against the purified recombinant FlaC protein and the reaction of the antibody was confirmed by western blot analysis using the FlaC protein and crude protein extracts of V. alginolyticus. Red snapper (Lutjanus sanguineus) vaccinated with recombinant FlaC produced specific antibodies, and were highly resistant to infection by virulent V. alginolyticus. This study indicates that the conserved FlaC is an effective vaccine candidate against V. alginolyticus infection. (C) 2010 Elsevier Ltd. All rights reserved.