Gene expression profiling of bovine mammary gland epithelial cells stimulated with lipoteichoic acid plus peptidoglycan from Staphylococcus aureus
Gene expression profiling of bovine mammary gland epithelial cells stimulated with lipoteichoic acid plus peptidoglycan from Staphylococcus aureus
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DOI：
10.1016/j.intimp.2014.05.002
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发表时间：
2014-07-01
影响因子：
5.6
通讯作者：
Han, Seung Hyun
中科院分区：
文献类型：
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作者：
Im, Jintaek;Lee, Taeheon;Han, Seung Hyun
已知革兰氏阳性细菌金黄色葡萄球菌是引起牛乳腺炎的主要病原菌之一。在S.金黄色葡萄球菌、脂磷壁酸（LTA）和肽聚糖（PGN）与炎症反应密切相关。而来源于S.金黄色葡萄球菌在牛乳腺炎中的作用尚未明确阐明。在这项研究中，我们的特点是牛乳腺上皮细胞系，MAC-T细胞的基因表达谱，在LTA和PGN的存在下，从S。金黄色。LTA加PGN，但不是单独的LTA或PGN，激活MAC-T细胞。使用Affyphin基因芯片微阵列的转录谱分析显示，用LTA加PGN刺激产生总共2019个（倍数变化>1.2）差异表达基因（DEG），其中801个上调基因和1218个下调基因。在表达上调的基因中，炎症介质相关的DEGs有14个，细胞内信号分子相关的DEGs有22个，转录因子相关的DEGs有15个，而在表达下调的DEGs中，炎症相关的DEGs有17个。这些结果提供了一个全面的分析基因表达谱引起的S。aureus LTA和PGN在MAC-T细胞中的表达，有助于理解S.金黄色葡萄球菌引起的牛乳房炎。(C)2014爱思唯尔有限公司版权所有。
A Gram-positive bacterium, Staphylococcus aureus is known to be one of the major pathogenic bacteria responsible for causing bovine mastitis. Among the various cell wall components of S. aureus, lipoteichoic acid (LTA) and peptidoglycan (PGN) are closely associated with inflammatory responses. However, the role of LTA and PGN derived from S. aureus in bovine mastitis has not been clearly elucidated. In this study, we characterized the gene expression profile of a bovine mammary gland epithelial cell line, MAC-T cells, in the presence of LTA and PGN from S. aureus. LTA plus PGN, but not LTA or PGN alone, activated MAC-T cells. The analysis of transcriptional profiles using an Affymetrix genechip microarray showed that stimulation with LTA plus PGN produced a total of 2019 (fold change >1.2) differentially expressed genes (DEGs), with 801 up-regulated genes and 1218 down-regulated genes. Of the up-regulated genes, 14 inflammatory mediator-related DEGs, 22 intra-cellular signaling molecule-related DEGs, and 15 transcription factor-related DEGs were observed, whereas among the down-regulated DEGs 17 inflammation-related DEGs were found. The microarray results were confirmed using real-time RT-PCR of 18 genes with substantial changes in expression (9 each from the up-regulated and down-regulated DEGs). These results provide a comprehensive analysis of gene-expression profiles elicited by S. aureus LTA and PGN in MAC-T cells, contributing to an understanding of the pathogenesis for S. aureus-induced bovine mastitis. (C) 2014 Elsevier B.V. All rights reserved.