Subunit structure of the mammalian exocyst complex
Subunit structure of the mammalian exocyst complex
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DOI：
10.1073/pnas.94.26.14438
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发表时间：
1997-12-23
影响因子：
11.1
通讯作者：
Scheller, RH
中科院分区：
文献类型：
--
作者：
Kee, Y;Yoo, JS;Scheller, RH
与广泛分布于靶膜上的重要分泌蛋白SNAREs （SNAP受体）不同，外囊特异性位于囊泡融合位点。我们已经分离出编码哺乳动物复合体的rexo70、rsec5和rsec15亚基的cdna。这些基因编码的氨基酸序列与酵母同源基因的同源性在21%至24%之间，这三个基因都广泛表达，并且在rexo70和rsec15中观察到多个转录本。对编码哺乳动物复合物84-kDa亚基的cdna进行表征发现了一种新的蛋白，并生成了针对哺乳动物胞囊复合物rsec6亚基的单克隆抗体。rsec6免疫反应性在PC12细胞突起末端或颗粒胞吐部位附近呈点状分布。
The exocyst is a protein complex required for the late stages of secretion in yeast, Unlike the SNAREs (SNAP receptors), important secretory proteins that are broadly distributed on the target membrane, the exocyst is specifically located at sites of vesicle fusion. me have isolated cDNAs encoding the rexo70, rsec5, and rsec15 subunits of the mammalian complex. The amino acid sequences encoded by these genes are between 21% and 24% identical to their yeast homologs, All three genes are broadly expressed and multiple transcripts are observed for rexo70 and rsec15, Characterization of cDNAs encoding the 84-kDa subunit of the mammalian complex revealed a novel protein, mAbs were generated to the mammalian rsec6 subunit of the exocyst complex, rsec6 immunoreactivity is found in a punctate distribution at terminals of PC12 cell processes at or near sites of granule exocytosis.