Dermatophagoides farinae Allergens Diversity Identification by Proteomics
Dermatophagoides farinae Allergens Diversity Identification by Proteomics
复制标题
蛋白质组学鉴定粉尘螨过敏原多样性
DOI：
10.1074/mcp.m112.027136
复制
发表时间：
2013-07-01
影响因子：
7
通讯作者：
Lai, Ren
中科院分区：
文献类型：
--
作者：
An, Su;Chen, Lingling;Lai, Ren
人类最重要的室内过敏原是屋尘螨（HDM）。粉尘螨（Dermatophagoides farinae）变应原有30多种，但已报道14种（Der f 1-3，6，7，10，11，1 - 3 -18和22）。花粉采用蛋白质组学结合二维免疫印迹技术，从D.淀粉提取物。通过Edman降解、质谱分析和cDNA克隆确定它们的序列。采用酶联免疫吸附抑制试验、免疫印迹法、嗜碱性粒细胞活化试验和皮肤点刺试验检测其致敏性。其中8个为首次报道的D.粉尘过敏原使用蛋白质组学方法结合纯粹的发现方法，使用具有广泛IgE反应谱的患者血清对螨过敏原的程序是研究更完整的D.粉尘过敏原鉴定了8个新D.粉尘类过敏原有助于HDM过敏的诊断和治疗，特别是对HDM主要过敏原无应答的患者。此外，目前的工作大大扩展了D.粉尘过敏原
The most important indoor allergens for humans are house dust mites (HDM). Fourteen Dermatophagoides farinae allergens (Der f 1-3, 6, 7, 10, 11, 13-18, and 22) are reported although more than 30 allergens have been estimated in D. farinae. Seventeen allergens belonging to 12 different groups were identified by a procedure of proteomics combined with two-dimensional immunoblotting from D. farina extracts. Their sequences were determined by Edman degradation, mass spectrometry analysis, and cDNA cloning. Their allergenicities were assayed by enzyme-linked immunosorbent assay inhibition tests, immunoblots, basophil activation test, and skin prick tests. Eight of them are the first report as D. farinae allergens. The procedure of using a proteomic approach combined with a purely discovery approach using sera of patients with broad IgE reactivity profiles to mite allergens was an effective method to investigate a more complete repertoire of D. farinae allergens. The identification of eight new D. farinae allergens will be helpful for HDM allergy diagnosis and therapy, especially for patients without response for HDM major allergens. In addition, the current work significantly extendedthe repertoire of D. farinae allergens.