Presence of Viral Genome in Urine and Development of Hematuria and Pathological Changes in Kidneys in Common Marmoset (Callithrix jacchus) after Inoculation with Dengue Virus.
Presence of Viral Genome in Urine and Development of Hematuria and Pathological Changes in Kidneys in Common Marmoset (Callithrix jacchus) after Inoculation with Dengue Virus.
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DOI：
10.3390/pathogens2020357
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发表时间：
2013-05-13
期刊：
影响因子：
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通讯作者：
Kurane I
中科院分区：
文献类型：
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作者：
Moi ML;Omatsu T;Hirayama T;Nakamura S;Katakai Y;Yoshida T;Saito A;Tajima S;Ito M;Takasaki T;Akari H;Kurane I
普通绒猴（Callithrix jacchus）在接种登革病毒（DENV）后发展出高水平的病毒血症，临床症状包括发热、体重减轻、活动减少和血尿。本研究检测了尿标本中DENV基因组的存在和肾脏的病理变化。在来自20只初次接种DENV的绒猴的228份尿液样品和来自4只二次接种DENV的绒猴的56份尿液样品中测定DENV基因组水平。感染后75%（15/20）的绒猴中检出DENV基因组。在继发感染DENV的绒猴尿液中未检测到DENV基因组（0%，0/4）。2只绒猴出现血尿。肾脏的病理学分析显示非抑制性间质性肾炎伴肾小管再生。在肾脏中检测到DENV抗原阳性细胞。在人类登革热病毒感染中，一些患者会出现肾脏症状。结果表明，绒猴概括了肾脏参与人类DENV感染的某些方面，并表明绒猴对DENV感染（包括肾脏）的发病机制的研究具有潜在的用途。
Common marmosets (Callithrix jacchus) developed high levels of viremia, clinical signs including fever, weight loss, a decrease in activity and hematuria upon inoculation with dengue virus (DENV). Presence of DENV genome in urine samples and pathological changes in kidneys were examined in the present study. Levels of DENV genome were determined in 228 urine samples from 20 primary DENV-inoculated marmosets and in 56 urine samples from four secondary DENV-inoculated marmosets. DENV genome was detected in 75% (15/20) of marmosets after primary DENV infection. No DENV genome was detected in urine samples from the marmosets with secondary infection with homologous DENV (0%, 0/4). Two marmosets demonstrated hematuria. Pathological analysis of the kidneys demonstrated non-suppressive interstitial nephritis with renal tubular regeneration. DENV antigen-positive cells were detected in kidneys. In human dengue virus infections, some patients present renal symptoms. The results indicate that marmosets recapitulate some aspects of the involvement of kidneys in human DENV infection, and suggest that marmosets are potentially useful for the studies of the pathogenesis of DENV infection, including kidneys.