Plasticity of adipose tissue in response to fasting and refeeding in male mice.
Plasticity of adipose tissue in response to fasting and refeeding in male mice.
复制标题
雄性小鼠禁食和再进食时脂肪组织的可塑性
DOI：
10.1186/s12986-016-0159-x
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发表时间：
2017
影响因子：
4.5
通讯作者：
Zhou HD
中科院分区：
文献类型：
--
作者：
Tang HN;Tang CY;Man XF;Tan SW;Guo Y;Tang J;Zhou CL;Zhou HD
背景禁食是治疗体重过度增加和肥胖症的最广泛的处方和自我强加的策略，并且已经显示出发挥许多有益的作用。方法C57/BL 6小鼠禁食24 ~ 72 h后，再喂食72 h，观察禁食和再喂食对小鼠脂肪分布的影响。在禁食24、48和72 h以及再喂食12、24、48和72 h时，处死小鼠，收集血清和各种脂肪组织。检测不同部位血清生化指标、脂肪组织重量及组织形态学变化。从各种脂肪组织中提取mRNA，并检测产热、内脏特征和脂代谢相关基因的表达。青少年和成年小鼠进行禁食和refeeding之间的脂肪组织的表型也compared.ResultsFasting优先消耗肠系膜脂肪质量和减少肠系膜贮库的细胞大小，但是，refeeding恢复腹股沟脂肪组织的质量和形态优先与内脏贮库相比。产热相关基因在腹股沟WAT和肩胛间BAT的表达受到抑制。线粒体生物合成的影响，禁食在特定的方式。此外，短期禁食导致皮下脂肪组织中内脏特征基因（Wt 1，Tcf 21）的增加，而这些基因的表达随着禁食时间的增加而急剧下降。此外，脂肪生成相关的标志物增强到更大的程度更大的皮下贮库相比，内脏脂肪组织的再喂养。虽然类似的表型变化，在脂肪组织之间观察到幼年小鼠和成年小鼠进行禁食和再喂养，改变出现更早，更敏感的幼年mice.ConclusionsFasting优先消耗内脏脂肪组织中的脂质，而再喂养主要是在皮下脂肪组织中回收脂质，这表明在食物剥夺或再喂养时，脂肪器官的可塑性对脂肪分布的重要性。
BackgroundFasting is the most widely prescribed and self-imposed strategy for treating excessive weight gain and obesity, and has been shown to exert a number of beneficial effects. The aim of the present study was to determine the exact role of fasting and subsequent refeeding on fat distribution in mice.MethodsC57/BL6 mice fasted for 24 to 72 h and were then subjected to refeeding for 72 h. At 24, 48 and 72 h of fasting, and 12, 24, 48 and 72 h of refeeding, the mice were sacrificed, and serum and various adipose tissues were collected. Serum biochemical parameters, adipose tissue masses and histomorphological analysis of different depots were detected. MRNA was isolated from various adipose tissues, and the expressions of thermogenesis, visceral signature and lipid metabolism-related genes were examined. The phenotypes of adipose tissues between juvenile and adult mice subjected to fasting and refeeding were also compared.ResultsFasting preferentially consumed mesenteric fat mass and decreased the cell size of mesenteric depots; however, refeeding recovered the mass and morphology of inguinal adipose tissues preferentially compared with visceral depots. Thermogenesis-related gene expression in the inguinal WAT and interscapular BAT were suppressed. Mitochondrial biogenesis was affected by fasting in a depot-specific manner. Furthermore, a short period of fasting led to an increase in visceral signature genes (Wt1, Tcf21) in subcutaneous adipose tissue, while the expression of these genes decreased sharply as the fasting time increased. Additionally, lipogenesis-related markers were enhanced to a greater extent greater in subcutaneous depots compared with those in visceral adipose tissues by refeeding. Although similar phenotypic changes in adipose tissue were observed between juvenile mice and adult mice subjected to fasting and refeeding, the alterations appeared earlier and more sensitively in juvenile mice.ConclusionsFasting preferentially consumes lipids in visceral adipose tissues, whereas refeeding recovers lipids predominantly in subcutaneous adipose tissues, which indicated the significance of plasticity of adipose organs for fat distribution when subject to food deprivation or refeeding.