High rate of mixoploidy among human blastocysts cultured in vitro
High rate of mixoploidy among human blastocysts cultured in vitro
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DOI：
10.1016/s0015-0282(02)04393-5
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发表时间：
2002-12-01
影响因子：
6.7
通讯作者：
Ao, A
中科院分区：
文献类型：
--
作者：
Bielanska, M;Tan, SL;Ao, A
目的：为了确定在体外生产的人类囊胚混合倍体的发病率和类型。设计：一个实验室研究的备用囊胚从体外受精程序。设置：大学医院实验室。患者（S）：39对夫妇接受体外受精或卵胞浆内单精子注射。干预（S）：共103个囊胚被归类为良好或不良质量的囊胚的基础上形态。共6,927个间期核，其中5,015个来自59个优质囊胚，1,912个来自44个劣质囊胚，通过染色体特异性DNA探针荧光原位杂交进行倍性评估。主要结果测量：每个囊胚中多倍体细胞的百分比和类型。在分析的86%的优质囊胚和82%的劣质囊胚中发现了混倍体（二倍体和多倍体细胞的混合物）。多倍体的类型从3 N到14 N不等，其中四倍体是两组之间最常见的。每个混倍体囊胚的多倍体细胞比例范围为1%至88%。在大多数优质混倍体囊胚中，多倍体核的比例很小（10%），显著低于劣质囊胚（19%）。在大多数优质囊胚中，多倍体细胞的比例很低。少量的囊胚和高比例的多倍体细胞可能对囊胚移植有影响。
Objective: To determine the incidence and type of mixoploidy in human blastocysts produced in vitro.Design: A laboratory study of spare blastocysts from an IVF program.Setting: University hospital laboratory.Patient(s): Thirty-nine couples undergoing IVF or intracytoplasmic sperm injection.Intervention(s): A total of 103 blastocysts were classified as good- or poor-quality blastocysts based on morphology. A total of 6,927 interphase nuclei, 5,015 from 59 good-quality and 1,912 from 44 poor-quality blastocysts, were assessed for ploidy by fluorescence in situ hybridization with chromosome-specific DNA probes.Main Outcome Measure(s): The percentage and the type of polyploid cells present in each blastocyst.Result(s): Mixoploidy (mixture of diploid and polyploid cells) was found in 86% of good-quality and 82% of poor-quality blastocysts analyzed. The type of polyploidy ranged from 3N to 14N, with tetraploidy being the most common between both groups. The proportion of polyploid cells per mixoploid blastocyst ranged from 1% to 88%. The percentage of polyploid nuclei within most good-quality mixoploid blastocysts was small (10%) and significantly lower than in poor-quality blastocysts (19%).Conclusion(s): Most human blastocysts produced in vitro contain polyploid, predominantly tetraploid cells. The proportion of polyploid cells in the majority of good-quality blastocysts is low. The small numbers of blastocysts with a high percentage of polyploid cells may have implications for blastocyst transfer.