Fat deposition in the tunica muscularis and decrease of interstitial cells of Cajal and nNOS-positive neuronal cells in the aged rat colon
Fat deposition in the tunica muscularis and decrease of interstitial cells of Cajal and nNOS-positive neuronal cells in the aged rat colon
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DOI：
10.1152/ajpgi.00304.2012
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发表时间：
2014-04-01
影响因子：
4.5
通讯作者：
Jung, Hyun Chae
中科院分区：
文献类型：
--
作者：
Jo, Hyun Jin;Kim, Nayoung;Jung, Hyun Chae
Jo HJ、Kim N、Nam RH、Kang JM、Kim J-H、Choe G、Lee HS、Park JH、Chang H、Kim H、Lee MY、Kim YS、Kim JS、Jung HC。老年大鼠结肠肌层脂肪沉积以及Cajal间质细胞和nNOS阳性神经元细胞减少。 Am J Physiol Gastrointest Liver Physiol 306：G659-G669，2014 年。首次发表于 2014 年 2 月 13 日； doi：10.1152/ajpgi。 00304.2012.-对于结肠卡哈尔间质细胞（ICC）衰老的时间进程知之甚少。本研究的目的是研究老年大鼠形态、ICC 和神经元一氧化氮合酶 (nNOS) 免疫反应细胞的变化。研究人员对 344 只 Fischer 大鼠在四个不同年龄（6、31、74 周和 2 岁）的近端结肠进行了研究。免疫组化后计数c-Kit、nNOS、抗蛋白基因产物9.5和突触素的免疫反应性。通过实时PCR测量c-kit、干细胞因子（Kit的配体）和nNOS mRNA。通过蛋白质印迹评估c-Kit和nNOS蛋白。进行等容收缩力测量和电场刺激（EFS）。 31周后，肌内脂肪沉积面积随着年龄的增长而显着增加。 c-Kit 免疫反应性 ICC 和 nNOS 免疫反应性神经元和神经纤维随着年龄的增长而显着下降。 c-kit和nNOS的mRNA和蛋白表达随着年龄的增长而降低。功能研究表明，老年大鼠的自发收缩力下降，而阿托品和L-NG-硝基精氨酸甲酯存在下老年大鼠的EFS反应增加。总之，衰老过程中固有平滑肌比例、ICC 和 nNOS 免疫反应神经元纤维密度以及 nNOS 免疫反应神经元数量的减少可能解释了与衰老相关的结肠运动障碍。
Jo HJ, Kim N, Nam RH, Kang JM, Kim J-H, Choe G, Lee HS, Park JH, Chang H, Kim H, Lee MY, Kim YS, Kim JS, Jung HC. Fat deposition in the tunica muscularis and decrease of interstitial cells of Cajal and nNOS-positive neuronal cells in the aged rat colon. Am J Physiol Gastrointest Liver Physiol 306: G659-G669, 2014. First published February 13, 2014; doi: 10.1152/ajpgi. 00304.2012.-Little is known about the time course of aging on interstitial cells of Cajal (ICC) of colon. The aim of this study was to investigate the change of morphology, ICC, and neuronal nitric oxide synthase (nNOS)-immunoreactive cells in the aged rat. The proximal colon of 344 Fischer rats at four different ages (6, 31, 74 wk, and 2 yr) were studied. The immunoreactivity of c-Kit, nNOS, anti-protein gene product 9.5, and synaptophysin were counted after immunohistochemistry. The c-kit, stem cell factor (ligand of Kit), and nNOS mRNA were measured by real-time PCR. c-Kit and nNOS protein were assessed by Western blot. Isovolumetric contractile force measurement and electrical field stimulation (EFS) were conducted. The area of intramuscular fat deposition significantly increased with age after 31 wk. c-Kit-immunoreactive ICC and nNOS-immunoreactive neurons and nerve fibers significantly declined with age. mRNA and protein expression of c-kit and nNOS decreased with aging. The functional study showed that the spontaneous contractility was decreased in aged rat, whereas EFS responses in the presence of atropine and L-NG-Nitroarginine methyl ester were increased in aged rat. In conclusion, the decrease of proportion of proper smooth muscle, the density of ICC and nNOS-immunoreactive neuronal fibers, and the number of nNOS-immunoreactive neurons during the aging process may explain the aging-associated colonic dysmotility.