Rabbit hemorrhagic disease virus (RHDV): Ultrastructure and biochemical studies of typical and core-like particles present in liver homogenates
Rabbit hemorrhagic disease virus (RHDV): Ultrastructure and biochemical studies of typical and core-like particles present in liver homogenates
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DOI：
10.1016/0168-1702(96)01285-3
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发表时间：
1996-04-01
期刊：
影响因子：
5
通讯作者：
Schirrmeier, H
中科院分区：
文献类型：
--
作者：
Granzow, H;Weiland, F;Schirrmeier, H
从急性风湿性心脏病兔身上分离出的杯状病毒颗粒与在慢性病兔身上发现的病毒颗粒进行了比较。迁延病兔肝匀浆无血凝活性，含有直径25-27 nm的病毒颗粒。这些病毒粒子只含有一种30 kDa的结构蛋白，明显小于完整的兔出血症病毒(RHDV)(32-40 mA)。为了证明较小病毒粒子的RHDV特性，通过病毒粒子蛋白的免疫印迹和免疫电子显微镜研究了它们与RHDV特异性抗体的反应性。RHDV颗粒经α-胰凝乳酶消化后，不能转化为较小的RHDV。我们认为这些核心样颗粒(CLP)不是蛋白质降解的结果，而是RHDV基因组截短或表达缺陷的结果。
Calicivirus particles isolated from rabbits suffering from acute RHD were compared with virions found in rabbits with chronic disease. Liver homogenates of rabbits with the protracted disease display no hemagglutinating activity and contain viral particles with diameters of 25-27 nm. These virions contain only one structural protein of 30 kDa and are distinctly smaller than intact rabbit hemorrhagic disease virus (RHDV) (32-40 ma). To prove the RHDV identity of the smaller virions, their reactivity with RHDV specific antibodies was investigated by immunoblots of the virion protein and by immunoelectron microscopy. Proteolytic digestion of RHDV particles with alpha-chymotrypsin did not transform RHDV into the smaller form. We assume that these core-like particles (CLPs) are not a result of proteolytic digestion but arise from a truncated RHDV genome or defective expression.