POROSITY OF YEAST-CELL WALL AND MEMBRANE
POROSITY OF YEAST-CELL WALL AND MEMBRANE
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DOI：
10.1128/jb.118.2.534-540.1974
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发表时间：
1974-01-01
影响因子：
3.2
通讯作者：
GERHARDT, P
中科院分区：
文献类型：
--
作者：
SCHERRER, R;LOUDEN, L;GERHARDT, P
通过一系列不同分子大小的惰性探测分子获得的被动平衡吸收值的三相模式的拐点，确定了能够渗透完整细胞壁和酿酒酵母原生质体膜的分子的极限大小。在酵母原生质体鉴定的阶段，吸收排斥阈值对应于分子量为110的单分散乙二醇，Einstein-Stokes流体动力半径（rES）为0.42 nm。在细胞壁期，阈值对应于数平均分子量（¯Mn） = 620，平均半径(rES) = 0.81 nm的多分散聚乙二醇。第三阶段对应于大分子的完全排除。细胞壁孔隙度的评估是通过使用第二种方法进行的，该方法涉及分析凝胶色谱分析单个多分散聚乙二醇被细胞摄取前后的分子量分布，表明细胞壁的准单分散阈值为mn = 760和res = 0.89 nm。这一结果与之前报道的两种情况一致，即更大的蛋白质分子能够穿透酵母细胞壁。
The limiting sizes of molecules that can permeate the intact cell wall and protoplast membrane ofSaccharomyces cerevisiaewere determined from the inflection points in a triphasic pattern of passive equilibrium uptake values obtained with a series of inert probing molecules varying in molecular size. In the phase identified with the yeast protoplast, the uptake-exclusion threshold corresponded to a monodisperse ethylene glycol of molecular weight = 110 and Einstein-Stokes hydrodynamic radius (rES) = 0.42 nm. In the cell wall phase, the threshold corresponded to a polydisperse polyethylene glycol of number-average molecular weight (¯Mn) = 620 and average radius (rES) = 0.81 nm. The third phase corresponded to complete exclusion of larger molecules. The assessment of cell wall porosity was confirmed by use of a second method involving analytical gel chromatographic analyses of the molecular weight distribution for a single polydisperse polyglycol before and after uptake by the cells, which indicated a quasi-monodisperse threshold for the cell wall ofMn= 760 andrES= 0.89 nm. The results were reconciled with two situations in which much larger protein molecules previously have been reported able to penetrate the yeast cell wall.