Prostaglandin and thromboxane synthesis by microsomes of rabbit ocular tissues.
Prostaglandin and thromboxane synthesis by microsomes of rabbit ocular tissues.
复制标题
兔眼组织微粒体合成前列腺素和血栓素。
DOI：
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发表时间：
1979
影响因子：
4.4
通讯作者：
N. Holmberg
中科院分区：
文献类型：
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作者：
M. Kass;N. Holmberg
将白化兔眼组织微粒体与(1-14C)-花生四烯酸在37℃下培养15 min，薄层色谱显示睫状体-虹膜微粒体能够合成前列腺素f2 - α (pgf2 α)、PGE2、PGD2、血栓素B2（TXB2）和6-酮- pgf1 α。吲哚美辛14微米在培养培养基中基本上消除了所有前列腺素的合成检测方法。咪唑10 mM仅抑制TXB2的合成。纤毛体-虹膜微粒体与PGH2在0℃下孵育2分钟。这种反应的产物被灌注在兔主动脉的螺旋条上，产生典型的TXA2的强烈收缩。在培养培养基中加入咪唑阻断了收缩物质的形成。纤毛体-虹膜微粒体与(1-14C)- 8,11,14-二十碳三烯酸孵育产生pgf1 α、PGD1和PGE1，但没有任何血栓素产物或6-酮- pgf1 α的证据。结膜和角膜微粒体在(1-14C)-花生四烯酸的作用下合成前列腺素，但效率低于睫状体-虹膜微粒体。巩膜、视网膜脉络膜和晶状体的微粒体即使有也很少合成前列腺素。
Microsomes of albino rabbit ocular tissues were incubated with (1-14C)-arachidonic acid for 15 min at 37 degrees C. Thin-layer chromatography revealed that ciliary body-iris microsomes were capable of synthesizing prostaglandin F2alpha (PGF2alpha), PGE2, PGD2, thromboxane B2(TXB2), and 6-keto-PGF1alpha. Indomethacin 14 micrometer in the incubation medium essentially abolished all prostaglandin synthesis detectable by this method. Imidazole 10 mM in the incubation medium inhibited only TXB2 synthesis. Ciliary body-iris microsomes were incubated for 2 min at 0 degrees C with PGH2. The products of this reaction were superfused over spiral strips of rabbit aorta and produced the strong contractions typical of TXA2. Addition to imidazole to the incubation medium blocked the formation of the contracting substance. Incubation of ciliary body-iris microsomes with (1-14C)--8,11,14-eicosatrienoic acid produced PGF1alpha, PGD1, and PGE1 but no evidence of any thromboxane product or 6-keto-PGF1alpha. Conjunctival and corneal microsomes synthesized prostaglandins, although less effectively than ciliary body-iris microsomes, when incubated with (1-14C)-arachidonic acid. Microsomes of sclera, retina-choroid, and lens synthesized little, if any, prostaglandins.