Label-free quantification of peptides in solution by disposable patterned hydrophilic chip based MALDI imaging
Label-free quantification of peptides in solution by disposable patterned hydrophilic chip based MALDI imaging
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DOI：
10.1016/j.cclet.2016.02.020
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发表时间：
2016-06
影响因子：
9.1
通讯作者：
Ting Wu;Xiaohui Yang;Chuanjing Zhang;Zhenping Wang;Yiping Du
中科院分区：
文献类型：
--
作者：
Ting Wu;Xiaohui Yang;Chuanjing Zhang;Zhenping Wang;Yiping Du
通过基于一次性图案化亲水芯片的基质辅助激光解吸/电离质谱成像（MALDI MSI）实现溶液中肽混合物的定量。与溶液中肽的其他定量方法相比，该方法是无标记的，并且在分析前不需要分离溶液的多种组分。均匀的亲水性斑点和高质量精度测量提供了可靠的识别和定量分析成像化合物。在5 fmol/μL ~ 1 pmol/μL范围内，四种多肽的浓度与图像灰度值呈线性相关。灵敏度高，重现性好。该方法将MALDI MSI的应用从组织扩展到溶液。
Quantification of a mixture of peptides in solution was achieved by disposable patterned hydrophilic chip based matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI MSI). Compared with other quantitative methods for peptides in solution, this method is label-free and does not require separation of the multiple components of the solution before analysis. Uniform hydrophilic spots and high mass accuracy measurements provided confident identification and quantitative analysis of imaged compounds. The linear correlation between concentration and grayscale of image in the range of 5 fmol/μL to 1 pmol/μL was obtained for all four peptides. Good sensitivity and excellent reproducibility were also achieved. The method expands the application of MALDI MSI from tissues to solutions.